Epand R M, Epand R F, Orlowski R C
Department of Biochemistry, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada.
Biochem Biophys Res Commun. 1988 Apr 15;152(1):203-7. doi: 10.1016/s0006-291x(88)80700-9.
A number of peptide hormones have been shown to contain amphipathic helical segments capable of binding to phospholipids. This conformational feature has been associated with increased biological activity of these hormones. We demonstrate, however, that two calcitonin analogs, [Gly8,Ala16]-des-Leu19 salmon calcitonin and des-1-amino-[Ala1,7,Gly8]-des-Leu19 salmon calcitonin have minimal interactions with phospholipids. Neither of these peptides acquire any increased helical content in the presence of dimyristoylphosphatidylglycerol and these peptides have only weak effects in altering the phase transition properties of this lipid. Therefore, although the presence of a phospholipid-induced amphipathic helical sequence may enhance the biological activity, it is not required for activity.
已证实多种肽类激素含有能够与磷脂结合的两亲性螺旋片段。这种构象特征与这些激素生物活性的增强有关。然而，我们证明，两种降钙素类似物，即[甘氨酸8，丙氨酸16]-去亮氨酸19鲑鱼降钙素和去1-氨基-[丙氨酸1,7，甘氨酸8]-去亮氨酸19鲑鱼降钙素与磷脂的相互作用极小。在二肉豆蔻酰磷脂酰甘油存在的情况下，这些肽均未获得任何增加的螺旋含量，并且这些肽在改变这种脂质的相变特性方面只有微弱的作用。因此，尽管磷脂诱导的两亲性螺旋序列的存在可能增强生物活性，但活性并不需要它。
