Hefei National Laboratory for Physical Sciences at Microscale, the CAS Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230027, China.
Chem Commun (Camb). 2021 Mar 14;57(21):2653-2656. doi: 10.1039/d0cc06508k. Epub 2021 Feb 15.
Here we present a novel peptide-based fluorescent "turn-on" molecule P1 for detecting RNA, in a double or single strand, AU-rich or CG-rich. Both computational and experimental studies indicate that the detection efficiency depends on the binding affinity of P1 and conformational changes. P1 could be applied for cell imaging without any additional transfection vectors. Selective detection of RNA in cells was determined by RNase digestion. Successful application of P1 for RNA imaging in cell mitosis reveals that it may have broad applications in research, biotechnology and medical science.
在这里，我们提出了一种新型的基于肽的荧光“开启”分子 P1，用于检测双链或单链、富含 AU 或富含 CG 的 RNA。计算和实验研究都表明，检测效率取决于 P1 的结合亲和力和构象变化。P1 可用于细胞成像，而无需任何额外的转染载体。通过 RNase 消化确定了 P1 在细胞中对 RNA 的选择性检测。P1 在细胞有丝分裂中的 RNA 成像的成功应用表明，它可能在研究、生物技术和医学科学中有广泛的应用。
