Department of Biological Sciences, University of Manitoba, 50 Sifton Road, Winnipeg, Manitoba, R3T 2N2, Canada.
Department of Biological Sciences, University of Manitoba, 50 Sifton Road, Winnipeg, Manitoba, R3T 2N2, Canada.
Fish Shellfish Immunol. 2021 May;112:1-7. doi: 10.1016/j.fsi.2021.02.007. Epub 2021 Feb 12.
Larval lake sturgeon, Acipenser fulvescens, reared in hatcheries for stock enhancement of wild populations may be susceptible to early opportunistic bacterial infection. Thus, we examined survival and whole-body mRNA expression of both stress- and immune-related genes (MyD88, IL-1β, StAR, GR1, and HSP70) in 30 days post fertilization larval lake sturgeon following immune challenge with lipopolysaccharides (LPS). Larval sturgeon were exposed to 0, 25, 50, 100, 150, and 200 μg ml LPS and sampled after 30 min, 4 h, and 48 h. Mortality was zero in 0 and 25 μg ml LPS; 37.5% in 50 μg ml LPS and 100% in the higher concentrations. Expression of MyD88 and StAR mRNA were positively correlated and increased with time in the 50 μg ml LPS treatment. There was an influence of both treatment and time on IL-1β mRNA, with expression 10-fold higher than controls after 4 h. Expression of HSP70 mRNA was suppressed within 30 min of 50 μg ml LPS exposure and remained so throughout the time course. Correlated mRNA expression of GR1 with MyD88, StAR and IL-1β suggests a potential relationship between the innate immune and glucocorticoid responses of larval lake sturgeon during this early developmental stage. Data presented suggest that larval lake sturgeon largely responded with predicted changes in gene expression of immune related and stress response genes following LPS challenge. This study provides a foundation for future research examining the effects of hatchery and naturally occurring stressors on the immune responses of larval lake sturgeon.
人工养殖的幼龄北美匙吻鲟（Acipenser fulvescens）可能容易受到早期机会性细菌感染，这些幼鱼是为了增加野生种群数量而在孵化场中培育的。因此，我们研究了脂多糖（LPS）免疫挑战后 30 天受精后幼龄北美匙吻鲟的存活情况和应激及免疫相关基因（MyD88、IL-1β、StAR、GR1 和 HSP70）的全身 mRNA 表达。幼鱼暴露于 0、25、50、100、150 和 200μg/ml LPS 中，分别在 30min、4h 和 48h 后取样。0 和 25μg/ml LPS 中的死亡率为 0；50μg/ml LPS 中的死亡率为 37.5%，而在更高浓度的 LPS 中死亡率为 100%。MyD88 和 StAR mRNA 的表达呈正相关，并随着时间的推移在 50μg/ml LPS 处理中增加。处理和时间均对 IL-1β mRNA 有影响，4h 后其表达水平是对照组的 10 倍。在 50μg/ml LPS 暴露后 30min，HSP70 mRNA 的表达被抑制，并且在整个时间过程中一直如此。GR1 的相关 mRNA 表达与 MyD88、StAR 和 IL-1β 相关，表明在这个早期发育阶段，幼龄北美匙吻鲟的先天免疫和糖皮质激素反应之间可能存在潜在关系。研究结果表明，幼龄北美匙吻鲟在 LPS 挑战后，其免疫相关和应激反应基因的表达与预期的变化大致一致。本研究为未来研究人工养殖和自然发生的应激源对幼龄北美匙吻鲟免疫反应的影响提供了基础。
