[利用简易发光计-ATP计研究生物活性物质对淋巴细胞能量代谢的影响]

[Effect of biologically active substances on lymphocyte energetics studied by using a simple luminometer-ATP meter].

作者信息

Larionov V N, Mokhova E N, Priorova N I, Tarakanova A N

出版信息

Nauchnye Doki Vyss Shkoly Biol Nauki. 1988(2):105-9.

Abstract

A method of lymphocyte energetics investigation according to the ATP concentration in cell suspension has been described. A simple easily reproducible luminometer was applied for ATP measurement by luminescence of luciferin/luciferase system. The conditions of cell incubation were found when the changes in mitochondrial metabolic state reflected on ATP concentration. For all this rotenone (5 nM) decreases the ATP concentration heavily than inhibits the rate of oxygen consumption. Ecto-ATPases hydrolyze quickly the low concentrations of exogenous ATP. The examples given show the possibilities of this method for studying the effect of biologically active substances on cell energetics.

摘要

已经描述了一种根据细胞悬液中ATP浓度来研究淋巴细胞能量代谢的方法。使用一种简单且易于重现的发光计，通过荧光素/荧光素酶系统的发光来测量ATP。发现了细胞孵育条件，此时线粒体代谢状态的变化反映在ATP浓度上。尽管如此，鱼藤酮（5 nM）比抑制氧气消耗速率更能大幅降低ATP浓度。胞外ATP酶能快速水解低浓度的外源性ATP。所举的例子展示了该方法在研究生物活性物质对细胞能量代谢影响方面的可能性。