Sentoukas Theodore, Demetzos Costas, Pispas Stergios
National Hellenic Research Foundation, Theoretical and Physical Chemistry Institute, Athens, Greece.
Laboratory of Pharmaceutical Nanotechnology, Department of Pharmacy, National and Kapodistrian University of Athens, Athens, Greece.
J Liposome Res. 2021 Sep;31(3):279-290. doi: 10.1080/08982104.2020.1806873. Epub 2021 Feb 16.
The purpose of this study is to prepare stimuli-responsive chimeric liposomes (i.e. hybrid polymer-lipid liposomes) containing functional copolymers and conduct aqueous solution studies in order to determine their properties and potential as drug-delivery nanocarriers. Two random copolymers, composed of the hydrophilic, pH and thermo-responsive 2-(dimethyl amino) ethyl methacrylate (DMAEMA) monomer and the hydrophobic stearyl methacrylate (SMA) monomer, were synthesized via free-radical polymerization and molecularly characterized using SEC, FTIR, and 1H-NMR. The synthesis was followed by aqueous solution studies, utilising dynamic light scattering (DLS) in order to determine their stimuli responsive self-assembly properties. The preparation of chimeric liposomes was mediated by thin film deposition and hydration, followed by aqueous solution studies via DLS, ζ-potential and fluorescence spectroscopy. The drug-loading studies include curcumin loading via a thin film deposition and hydration technique, while aqueous solution properties of the drug-loaded chimeric liposomes were determined utilizing DLS, and UV-Vis spectroscopy.
本研究的目的是制备含有功能共聚物的刺激响应性嵌合脂质体（即杂化聚合物-脂质脂质体），并进行水溶液研究，以确定其作为药物递送纳米载体的性质和潜力。通过自由基聚合合成了两种由亲水性、pH和温度响应性的甲基丙烯酸2-(二甲基氨基)乙酯（DMAEMA）单体和疏水性甲基丙烯酸硬脂酯（SMA）单体组成的无规共聚物，并使用尺寸排阻色谱（SEC）、傅里叶变换红外光谱（FTIR）和核磁共振氢谱（1H-NMR）对其进行了分子表征。合成之后进行水溶液研究，利用动态光散射（DLS）来确定其刺激响应性自组装性质。嵌合脂质体的制备通过薄膜沉积和水化介导，随后通过DLS、ζ电位和荧光光谱进行水溶液研究。载药研究包括通过薄膜沉积和水化技术负载姜黄素，同时利用DLS和紫外可见光谱确定载药嵌合脂质体的水溶液性质。
