Institute of Pathology, Ulm University, Ulm, Germany.
Ulm University Medical Center, Institute of Human Genetics, Ulm University, Ulm, Germany.
Br J Haematol. 2021 Apr;193(2):369-374. doi: 10.1111/bjh.17193. Epub 2021 Feb 16.
Extra-nodal marginal zone B-cell lymphoma (MZBL) of mucosa-associated lymphoid tissue is an indolent lymphoma mostly affecting the gastrointestinal tract. The lymphoma initially has small-cell morphology (SC-MZBL) and often arises in the background of Helicobacter pylori-induced gastritis. In some cases, a clonal malignant progression to large-cell morphology (LC-MZBL) is observed. Here, we studied the DNA methylation profile of 30 gastric MZBLs along their progression. Genome-wide DNA methylation profiling, identified 7698 significantly differentially methylated loci during gastric MZBL progression (σ/σ ≥0·4, q ≤ 0·001). LC-MZBL showed hypermethylation in comparison to SC-MZBL with an enrichment of regions involved in transcriptional regulation. In conclusion, our present data show that the morphological distinction between SC- and LC-MZBL is reflected by characteristic DNA methylation profiles.
结外黏膜相关边缘区 B 细胞淋巴瘤（MZBL）是一种惰性淋巴瘤，主要影响胃肠道。该淋巴瘤最初具有小细胞形态（SC-MZBL），并且经常在幽门螺杆菌引起的胃炎背景下发生。在某些情况下，观察到向大细胞形态（LC-MZBL）的克隆恶性进展。在这里，我们研究了 30 例胃 MZBL 在其进展过程中的 DNA 甲基化谱。全基因组 DNA 甲基化分析，在胃 MZBL 进展过程中鉴定出 7698 个显著差异甲基化位点（σ/σ≥0·4，q≤0·001）。与 SC-MZBL 相比，LC-MZBL 表现出过度甲基化，并且富含参与转录调控的区域。总之，我们目前的数据表明，SC-MZBL 和 LC-MZBL 之间的形态学区别反映在特征性的 DNA 甲基化谱中。
