全身暴露于 1GeV/n Ti 离子的雄性 CBA/Ca 小鼠骨髓细胞的早期和晚期损伤。
Early- and late-occurring damage in bone marrow cells of male CBA/Ca mice exposed whole-body to 1 GeV/n Ti ions.
机构信息
Pathology Department, Stony Brook University, Stony Brook, NY, USA.
Department of Chemical Engineering, Faculty of Engineering, Burapha University, Chonburi, Thailand.
出版信息
Int J Radiat Biol. 2021;97(4):517-528. doi: 10.1080/09553002.2021.1884312. Epub 2021 Mar 8.
PURPOSE
To determine the early- and late-occurring damage in the bone marrow (BM) and peripheral blood cells of male CBA/Ca mice after exposure to 0, 0.1, 0.25, or 0.5 Gy of 1 GeV/n titanium (Ti) ions (one type of space radiation).
METHOD
We used the mouse in vivo blood-erythrocyte micronucleus (MN) assay for evaluating the cytogenetic effects of various doses of 1 GeV/n Ti ions. The MN assay was coupled with the characterization of epigenetic alterations (the levels of global 5-methylcytosine and 5-hydroxymethylcytosine) in DNA samples isolated from BM cells. These analyses were performed in samples collected at an early time-point (1 week) and a late time-point (6 months) post-irradiation.
RESULTS
Our results showed that Ti ions induced genomic instability in exposed mice. Significant dose-dependent loss of global 5-hydroxymethylcytosine was found but there were no changes in global 5-methylcytosine levels.
CONCLUSION
Since persistent genomic instability and loss of global 5-hydroxymethylcytosine are linked to cancer, our findings suggest that exposure to Ti ions may pose health risks.
目的
确定雄性 CBA/Ca 小鼠在暴露于 0、0.1、0.25 或 0.5Gy1GeV/n 钛（Ti）离子（一种空间辐射）后骨髓（BM）和外周血细胞中早期和晚期发生的损伤。
方法
我们使用小鼠体内血红细胞微核（MN）测定法来评估各种剂量的 1GeV/n Ti 离子的细胞遗传学效应。MN 测定法与 DNA 样本中表观遗传改变（全基因组 5-甲基胞嘧啶和 5-羟甲基胞嘧啶水平）的特征相结合，这些样本取自 BM 细胞，分别在辐照后 1 周和 6 个月的早期和晚期时间点进行收集。
结果
我们的结果表明，Ti 离子诱导了暴露小鼠的基因组不稳定性。发现全基因组 5-羟甲基胞嘧啶的剂量依赖性显著丢失，但全基因组 5-甲基胞嘧啶水平没有变化。
结论
由于持续的基因组不稳定性和全基因组 5-羟甲基胞嘧啶的丢失与癌症有关，我们的研究结果表明，暴露于 Ti 离子可能会带来健康风险。
Zotero 插件