丙型肝炎病毒 RNA 转录本与非人乳头瘤病毒相关的头颈部鳞状细胞癌的预后相关。
Hepatitis C Virus RNA Transcript Associates with Prognosis in Non-human Papillomavirus Associated Head and Neck Squamous Cell Carcinoma.
机构信息
Department of Otolaryngology-Head and Neck Surgery, The Xiangya Hospital, Central South University, Changsha, China.
Department of Otolaryngology-Head and Neck Surgery, The Second Xiangya Hospital, Central South University, Changsha, China.
出版信息
Laryngoscope. 2021 Aug;131(8):1774-1781. doi: 10.1002/lary.29422. Epub 2021 Feb 16.
OBJECTIVE/HYPOTHESIS: Hepatitis C virus (HCV) was reported to associate with head and neck squamous cell carcinoma (HNSCC) in many studies. However, its correlation with prognosis of non-human papillomavirus (HPV) associated HNSCC remains unknown. Here, we sought to investigate clinical significance of HCV RNA transcript in non-HPV associated HNSCC by analyzing corresponding RNA-seq data.
STUDY DESIGN
A retrospective cohort study.
METHODS
Four hundred and forty-eight non-HPV associated HNSCC patients with aligned RNA-seq and clinical follow-up data were included and divided into two groups: low-HCV and high-HCV. Means of continuous variables and proportions of categorical variables were compared using independent sample t-test and chi-square test, respectively. Survival data were compared using Cox regression analysis, Kaplan-Meier curves, and log-rank test. Expression of genome-wide mRNAs and abundance of immune cells were compared using volcano plot and cell signature estimated score analysis.
RESULTS
HCV RNA transcript negatively correlates with pathologic (P = .028) and clinical-stage (P = .023), clinical N stage (P = .025), and nodal extracapsular spread (P = .042) and is an independent prognosis factor in non-HPV associated HNSCC (HR = 1.488; 95% CI: 1.004-2.206; P = .048). Elevated expression of HCV improved 5-year overall survival (43.6% vs. 53.2%; P = .035) in all non-HPV associated HNSCC patients, the same as in male (46.6% vs. 58.7%; P = .049), clinical M0 stage (42.8% vs. 52.9%; P = .036), white (42.9% vs. 55.9%; P = .010), and histologic grade 1 to 2 subgroups (42.1% vs. 57.2%; P = .043). The expression of several immune-related genes and abundance of some immune cells significantly changed with the increase of HCV RNA transcript, while HCV-related oncogenes and tumor suppressor gene did not.
CONCLUSIONS
HCV RNA transcript is an independent favorable factor for prognosis of non-HPV associated HNSCC.
LEVELS OF EVIDENCE
4 Laryngoscope, 131:1774-1781, 2021.
目的/假设：许多研究报道丙型肝炎病毒（HCV）与头颈部鳞状细胞癌（HNSCC）有关。然而，其与非人乳头瘤病毒（HPV）相关的 HNSCC 预后的相关性尚不清楚。在这里，我们通过分析相应的 RNA 测序数据，旨在研究 HCV RNA 转录物在非 HPV 相关 HNSCC 中的临床意义。
研究设计
回顾性队列研究。
方法
纳入了 448 例非 HPV 相关 HNSCC 患者，这些患者具有对齐的 RNA 测序和临床随访数据，并分为两组：低 HCV 和高 HCV。使用独立样本 t 检验和卡方检验分别比较连续变量和分类变量的平均值。使用 Cox 回归分析、Kaplan-Meier 曲线和对数秩检验比较生存数据。使用火山图和细胞特征估计评分分析比较全基因组 mRNA 的表达和免疫细胞的丰度。
结果
HCV RNA 转录物与病理分期（P=0.028）、临床分期（P=0.023）、临床 N 分期（P=0.025）和淋巴结外膜扩散（P=0.042）呈负相关，是非 HPV 相关 HNSCC 的独立预后因素（HR=1.488；95%CI：1.004-2.206；P=0.048）。HCV 高表达可改善所有非 HPV 相关 HNSCC 患者的 5 年总生存率（43.6% vs. 53.2%；P=0.035），在男性（46.6% vs. 58.7%；P=0.049）、临床 M0 期（42.8% vs. 52.9%；P=0.036）、白人（42.9% vs. 55.9%；P=0.010）和组织学分级 1-2 亚组（42.1% vs. 57.2%；P=0.043）中也是如此。随着 HCV RNA 转录物的增加，一些免疫相关基因的表达和一些免疫细胞的丰度显著改变，而 HCV 相关致癌基因和肿瘤抑制基因则没有。
结论
HCV RNA 转录物是非 HPV 相关 HNSCC 预后的独立有利因素。
证据水平
4 级喉镜，131：1774-1781，2021 年。
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