Department of Environmental Engineering, Yonsei University, Wonju, 26493, Republic of Korea.
Department of Environmental Engineering, Yonsei University, Wonju, 26493, Republic of Korea.
Environ Res. 2021 Apr;195:110878. doi: 10.1016/j.envres.2021.110878. Epub 2021 Feb 13.
Significant concentrations of pharmaceuticals and personal care products (PPCPs) have been detected in aquatic environment. Fungal enzymatic processes can oxidize these persistent PPCPs; thus, these processes have attracted considerable attention from the scientific community. Here, we evaluated the efficacy of the removal of PPCPs using native fungal enzymes derived from Bjerkandera spp. TBB-03 under various conditions. Among the eight lignocellulosic substrates, ash, which showed the highest laccase production, was selected as the sole enzyme inducer. TBB-03 laccase was found to exhibit remarkable stability under varied pH and temperature conditions. Acetaminophen and bisphenol A were effectively removed by TBB-03 laccase under various conditions, except at pH 8. Although TBB-03 laccase could not efficiently remove single-state sulfamethoxazole directly, a 22% of improvement in sulfamethoxazole removal was observed in the presence of acetaminophen. Overall, our proposed approach showed that Bjerkandera adusta TBB-03 can be potentially applied for further research regarding PPCP remediation.
大量的药品和个人护理产品（PPCPs）已在水生环境中被检出。真菌酶促过程可以氧化这些持久性 PPCPs；因此，这些过程引起了科学界的广泛关注。在这里，我们评估了使用源自 Bjerkandera spp. 的天然真菌酶（TBB-03）在各种条件下去除 PPCPs 的效果。在八种木质纤维素底物中，灰烬显示出最高的漆酶产量，被选为唯一的酶诱导剂。TBB-03 漆酶在不同的 pH 和温度条件下表现出显著的稳定性。TBB-03 漆酶在各种条件下都能有效地去除对乙酰氨基酚和双酚 A，但在 pH 值为 8 时除外。虽然 TBB-03 漆酶不能直接有效地去除单态磺胺甲恶唑，但在存在对乙酰氨基酚的情况下，磺胺甲恶唑的去除率提高了 22%。总的来说，我们提出的方法表明，Bjerkandera adusta TBB-03 可以潜在地应用于进一步研究 PPCP 的修复。
