Disciplina de Obstetricia, Departamento de Obstetricia e Ginecologia, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo, Brazil.
Departamento de Patologia, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo, Brazil.
J Gynecol Obstet Hum Reprod. 2021 May;50(5):102096. doi: 10.1016/j.jogoh.2021.102096. Epub 2021 Feb 13.
This study investigated the qualitative and semi-quantitative expression of metalloproteinases (MMP) and their tissue inhibitors (TIMP) in trophoblastic tissue during ampullary ectopic pregnancies and correlated that expression with the degree of tubal invasion.
It is a prospective study that included 34 patients diagnosed with ampullary tubal pregnancy who underwent salpingectomy. A histological evaluation of the depth of trophoblastic invasion in the tubes obtained was performed. Subsequently, the expression of the MMP-2, MMP-9, MMP-14, TIMP-1, TIMP-2 and TIMP-3 markers was qualitatively and semi-quantitatively evaluated by indirect immunohistochemistry. In addition, the degree of trophoblastic invasion was correlated with the expression of each marker and with the metalloproteinase/inhibitor ratios.
MMP-2 (11.2 %; 3.6-17.9) was the marker with greater expression at the implantation site, both in the qualitative and semi-quantitative assessment, while MMP-9 (2.23 %; 0.2-5.4) and TIMP-3 (2.53 %; 0.1-15.3) were only weakly expressed.
There was wide variation in expression among the markers and metalloproteinase/inhibitor ratios studied compared to the degrees of invasion.
本研究调查了金属蛋白酶（MMP）及其组织抑制剂（TIMP）在壶腹部异位妊娠滋养层组织中的定性和半定量表达，并将其与输卵管侵袭程度相关联。
这是一项前瞻性研究，共纳入 34 例接受输卵管切除术的壶腹部输卵管妊娠患者。对获得的输卵管中滋养层浸润的深度进行组织学评估。随后，通过间接免疫组织化学法对 MMP-2、MMP-9、MMP-14、TIMP-1、TIMP-2 和 TIMP-3 标志物进行定性和半定量评估。此外，还将滋养层浸润程度与每个标志物的表达以及金属蛋白酶/抑制剂的比值相关联。
在植入部位，MMP-2（11.2%；3.6-17.9）的表达无论是定性还是半定量评估都最高，而 MMP-9（2.23%；0.2-5.4）和 TIMP-3（2.53%；0.1-15.3）的表达则较弱。
与侵袭程度相比，研究中标记物和金属蛋白酶/抑制剂的比值表达存在很大差异。
