鉴定美洲豚草花粉中的一种变应原钙调蛋白。
Identification of an allergenic calmodulin from Amaranthus palmeri pollen.
机构信息
Sección De Estudios De Posgrado e Investigación, ESM, Instituto Politécnico Nacional, Plan De San Luis y Díaz Mirón s/n, Col. Casco De Santo Tomas, Alcaldía Miguel Hidalgo, C.P. 11340, Ciudad De México, Mexico; Sección de Estudios de Posgrado e Investigación, ENMH, Instituto Politécnico Nacional, Guillermo Massieu Helguera No. 239, Col. Frac. "La Escalera", Ticomán, Gustavo A. Madero, C.P. 07320, Ciudad De México, Mexico.
Sección de Estudios de Posgrado e Investigación, ENMH, Instituto Politécnico Nacional, Guillermo Massieu Helguera No. 239, Col. Frac. "La Escalera", Ticomán, Gustavo A. Madero, C.P. 07320, Ciudad De México, Mexico.
出版信息
Mol Immunol. 2021 Apr;132:150-156. doi: 10.1016/j.molimm.2021.01.031. Epub 2021 Feb 13.
INTRODUCTION
Pollens are an important source of allergens that trigger rhinitis or asthma. The allergenic extracts of pollens used to diagnose and treat allergies contain different allergenic antigens. Isolated allergenic proteins are employed in in vitro assays, skin tests and allergenic-specific immunotherapy. Calcium-binding allergens are clinically relevant antigens, and their allergenicity can be affected by Ca binding. In this work, a calmodulin was identified as an allergen from Amaranthus palmeri pollen, an important source of pollinosis in Europe, Asia and North America.
MATERIALS AND METHODS
Allergenic calmodulin from A. palmeri pollen was isolated by size-exclusion chromatography and reverse-phase chromatography and identified by mass spectrometry. Sensitization to isolated calmodulin was evaluated by skin prick tests in patients with allergy to A. palmeri pollen.
RESULTS
Size-exclusion chromatography yielded two fractions that were recognized by the IgE of patients allergic to A. palmeri pollen. Mass spectrometry analysis of the fractions from reverse-phase chromatography showed peptide sequences that identified a calmodulin. Skin prick tests showed that the isolated calmodulin was recognized by 56% of patients allergic to A. palmeri pollen.
CONCLUSION
A. palmeri pollen calmodulin could be a clinically relevant allergen in patients sensitized to this source.
简介
花粉是引发鼻炎或哮喘的重要过敏原来源。用于诊断和治疗过敏的花粉变应原提取物含有不同的变应原抗原。分离的变应原蛋白用于体外检测、皮肤试验和变应原特异性免疫治疗。钙结合变应原是具有临床相关性的抗原，其变应原性可能受到 Ca 结合的影响。在这项工作中，从美洲豚草花粉中鉴定出一种钙调蛋白，美洲豚草花粉是欧洲、亚洲和北美的重要花粉过敏来源。
材料和方法
通过大小排阻色谱和反相色谱从 A. palmeri 花粉中分离出变应原钙调蛋白，并通过质谱鉴定。通过对花粉过敏的患者进行皮肤点刺试验评估对分离的钙调蛋白的致敏情况。
结果
大小排阻色谱产生了两个被花粉过敏患者 IgE 识别的级分。反相色谱级分的质谱分析显示了鉴定出钙调蛋白的肽序列。皮肤点刺试验显示，分离的钙调蛋白被 56%的花粉过敏患者识别。
结论
致敏于这种花粉源的患者中，美洲豚草花粉钙调蛋白可能是一种具有临床相关性的变应原。
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