Raji Muhabat Adeola, Chinnappan Raja, Shibl Atef, Suaifan Ghadeer, Weber Karina, Cialla-May Dana, Popp Jürgen, El Shorbagy Enas, Al-Kattan Khaled, Zourob Mohammed
Department of Microbiology and Immunology, College of Medicine, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Rd, Riyadh, 11533, Saudi Arabia.
Department of Chemistry, Alfaisal University, Al Zahrawi Street, Al Maather, Al Takhassusi Rd, Riyadh, 11533, Saudi Arabia.
Talanta. 2021 Apr 1;225:121946. doi: 10.1016/j.talanta.2020.121946. Epub 2020 Dec 1.
The timely diagnosis of MRSA in clinical samples helps to reduce the attendant morbidity/mortality associated with infection due to the organism. The early institution of appropriate therapy or deployment of infection control protocols are dependent on a timely report from the microbiology laboratory. Various assays currently used in the identification of MRSA are associated with inherent shortcomings, thus there is a need to explore newer diagnostic frontiers that can eliminate some of these short comings at a relatively cheap, timely, specific and sensitive manner. We present in this study a MRSA specific optical immunosensor to detect the presence of the pathogen on contaminated surface using control and patient strains. Results revealed a detection limits of 10 CFU mL-1 upon visual observation, and 29 CFU mL-1 as determined by the linear regression equation, following the use of ImageJ to quantify activated cotton swab color intensity. The specificity of the sensor was examined by blind testing a panel of non-MRSA bacteria (E. coli, S. aureus and S. epidermis). Negative visual read-out was observed for all tested non-specific bacteria except for MRSA. Assay takes an average of 5 min and presents a powerful point-of-care diagnostic platform for the detection of MRSA.
临床样本中耐甲氧西林金黄色葡萄球菌（MRSA）的及时诊断有助于降低该病原体感染所致的发病率/死亡率。适当治疗的早期实施或感染控制方案的部署依赖于微生物实验室的及时报告。目前用于鉴定MRSA的各种检测方法都存在固有缺陷，因此有必要探索更新的诊断前沿技术，以相对廉价、及时、特异且灵敏的方式消除其中一些缺陷。在本研究中，我们展示了一种MRSA特异性光学免疫传感器，用于使用对照菌株和患者菌株检测污染表面上病原体的存在。结果显示，在使用ImageJ量化活化棉签颜色强度后，通过视觉观察检测限为10 CFU mL-1，根据线性回归方程确定为29 CFU mL-1。通过对一组非MRSA细菌（大肠杆菌、金黄色葡萄球菌和表皮葡萄球菌）进行盲测来检验传感器的特异性。除MRSA外，所有测试的非特异性细菌均观察到阴性视觉读数。该检测平均需要5分钟，为MRSA的检测提供了一个强大的即时诊断平台。
