Department of Bioengineering, Faculty of Engineering and Architecture, Konya Food and Agriculture University, 42080 Konya, Turkey; Research and Development Center for Diagnostic Kits (KIT-ARGEM), Konya Food and Agriculture University, 42080 Konya, Turkey.
Department of Bioengineering, Faculty of Engineering and Architecture, Konya Food and Agriculture University, 42080 Konya, Turkey; Research and Development Center for Diagnostic Kits (KIT-ARGEM), Konya Food and Agriculture University, 42080 Konya, Turkey.
Carbohydr Polym. 2021 Apr 15;258:117699. doi: 10.1016/j.carbpol.2021.117699. Epub 2021 Jan 27.
Herein, we present a study on the catalytic evaluation of biocompatible chitosan-stabilized gold nanoparticles (CH-AuNPs) on the oxidation of morin as a model reaction. Biocompatible CH-AuNPs have been characterized through several analytical methods such as TEM, UV-vis, DLS and zeta potential analyses. CH-AuNPs have a small size (10 ± 0.4 nm) with a narrow size distribution and high positive surface charge (+40.1 mV). CH-AuNPs has been demonstrated to be highly active nanocatalysts for the oxidation of morin with the assistance of HO as an oxidant compared with control experiments. The oxidation reaction follows a pseudo-first-order reaction. The kinetic studies show that apparent rate constant (k) is positively correlated with the concentrations of CH-AuNPs and HO, while it is negatively correlated with morin concentration. Furthermore, the reusability tests have been performed and the results demonstrate the long-term stability and reusability of CH-AuNPs without any loss of catalytic activity. Cytotoxicity studies exhibit that CH-AuNPs have low toxicity and they are biocompatible with HeLa and MCF-7 cells.
在此，我们研究了生物相容性壳聚糖稳定的金纳米粒子（CH-AuNPs）在作为模型反应的桑色素氧化中的催化评估。通过 TEM、UV-vis、DLS 和zeta 电位分析等几种分析方法对生物相容性 CH-AuNPs 进行了表征。CH-AuNPs 的尺寸较小（10±0.4nm），具有较窄的尺寸分布和较高的正表面电荷（+40.1mV）。与对照实验相比，CH-AuNPs 在 HO 作为氧化剂的协助下，被证明是桑色素氧化的高效纳米催化剂。氧化反应遵循准一级反应。动力学研究表明，表观速率常数（k）与 CH-AuNPs 和 HO 的浓度呈正相关，而与桑色素的浓度呈负相关。此外，还进行了可重复使用性测试，结果表明 CH-AuNPs 具有长期稳定性和可重复使用性，而没有任何催化活性损失。细胞毒性研究表明，CH-AuNPs 毒性低，与 HeLa 和 MCF-7 细胞生物相容性好。
