Laboratory of Food Safety and Food Integrity, Institute of Tropical Agriculture and Food Security, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Molecules. 2019 Aug 29;24(17):3141. doi: 10.3390/molecules24173141.
This work presents a simple green synthesis of gold nanoparticles (AuNPs) by using an aqueous extract of (torch ginger). The metabolites present in , including sugars, proteins, polyphenols, and flavonoids, were known to play important roles in reducing metal ions and supporting the subsequent stability of nanoparticles. The present work aimed to investigate the ability of the extract to synthesise AuNPs via the reduction of gold (III) chloride hydrate and characterise the properties of the nanoparticles produced. The antioxidant properties of the extract were evaluated by analysing the total phenolic and total flavonoid contents. To ascertain the formation of AuNPs, the synthesised particles were characterised using the ultraviolet-visible (UV-Vis) spectroscopy, Fourier transform infrared (FTIR) spectroscopy, high-resolution transmission electron microscopy (HRTEM), energy-dispersive X-ray (EDX) microscopy, and dynamic light scattering (DLS) measurement. The properties of the green synthesised AuNPs were shown to be comparable to the AuNPs produced using a conventional reducing agent, sodium citrate. The UV-Vis measured the surface plasmon resonance of the AuNPs, and a band centered at 529 nm was obtained. The FTIR results proved that the extract contained the O-H functional group that is responsible for capping the nanoparticles. The HRTEM images showed that the green synthesized AuNPs were of various shapes and the average of the nanoparticles' hydrodynamic diameter was 31.5 ± 0.5 nm. Meanwhile, the zeta potential of -32.0 ± 0.4 mV indicates the high stability and negative charge of the AuNPs. We further successfully demonstrated that using the green synthesised AuNPs as the nanocomposite to modify the working surface of screen-printed carbon electrode (SPCE/Cs/AuNPs) enhanced the rate of electron transfer and provided a sensitive platform for the detection of Cu(II) ions.
这项工作提出了一种通过使用（火炬姜）的水提物简单绿色合成金纳米粒子（AuNPs）的方法。 存在于 中的代谢物，包括糖，蛋白质，多酚和类黄酮，已知在还原金属离子和支持随后纳米粒子的稳定性方面发挥重要作用。 本工作旨在研究 提取物通过还原三氯化金水合物合成 AuNPs 的能力，并表征所产生的纳米粒子的性质。 通过分析总酚和总类黄酮含量来评估 提取物的抗氧化性能。 为了确定 AuNPs 的形成，使用紫外-可见（UV-Vis）光谱，傅里叶变换红外（FTIR）光谱，高分辨率透射电子显微镜（HRTEM），能量色散 X 射线（EDX）显微镜和动态光散射（DLS）测量对合成的颗粒进行了表征。 所制备的金纳米粒子的性质与使用常规还原剂柠檬酸钠制备的金纳米粒子相当。 UV-Vis 测量了 AuNPs 的表面等离子体共振，并且获得了中心位于 529nm 的带。 FTIR 结果证明，提取物含有负责包覆纳米粒子的 O-H 官能团。 HRTEM 图像显示，绿色合成的 AuNPs 具有各种形状，纳米粒子的平均水动力直径为 31.5±0.5nm。 同时，-32.0±0.4mV 的 ζ 电位表明 AuNPs 的高稳定性和带负电荷。 我们进一步成功地证明，使用绿色合成的 AuNPs 作为纳米复合材料来修饰丝网印刷碳电极（SPCE/Cs/AuNPs）的工作表面，增强了电子转移速率，并为 Cu（II）离子的检测提供了灵敏的平台。
