新型五味子多糖的结构特征及其对 PCB 免疫毒性的营养干预。
Structural characterization of a novel Schisandra polysaccharides and nutritional intervention in immunotoxicity to PCBs.
机构信息
School of Chemistry and Chemical Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang, 212013, China.
School of Food and Biological Engineering, Jiangsu University, Xuefu Rd. 301, Zhenjiang, 212013 Jiangsu, China.
出版信息
Carbohydr Polym. 2021 Apr 15;258:117380. doi: 10.1016/j.carbpol.2020.117380. Epub 2021 Jan 20.
A new polysaccharide from fruits of Schisandra chinensis (SCPP22) with a molecular weight of 143 ± 0.13 KDa was mainly made up of glucose and galactose. The possible structure of SCPP22 was showed that its main chain was composed of 1,4-α-d-Glup and branch was stretched from O-6 position of 1,4-β-d-Glup. Branches consisted of T-α-d-Galp. Further, SCPP22 could reverse PCB126-induced immunosuppression, significantly enhance body weight and immune organ indices. It also significantly ameliorated oxidative injury to immune organ induced by PCB126, as shown by evaluation of SOD activities, as well as MDA levels in spleen and thymus. SCPP22 strongly stimulated cytokines production by up-regulating mRNA expression of TNF-α, INF-γ and IL-2. Mechanism investigation revealed that recovery effects of SCPP22 in immunosuppression induced by PCB126 are mainly through regulating apoptosis-related proteins expression. Schisandra polysaccharides might be applied in functional food as nutritional intervention ingredient.
从五味子果实中提取得到一种新的多糖（SCPP22），其分子量为 143 ± 0.13 KDa，主要由葡萄糖和半乳糖组成。SCPP22 的可能结构表明，其主链由 1,4-α-d-Glup 组成，支链从 1,4-β-d-Glup 的 O-6 位伸展。支链由 T-α-d-Galp 组成。此外，SCPP22 可逆转 PCB126 诱导的免疫抑制，显著增加体重和免疫器官指数。它还显著改善了 PCB126 诱导的免疫器官氧化损伤，如 SOD 活性和脾、胸腺 MDA 水平的评估所示。SCPP22 通过上调 TNF-α、INF-γ 和 IL-2 的 mRNA 表达强烈刺激细胞因子的产生。机制研究表明，SCPP22 对 PCB126 诱导的免疫抑制的恢复作用主要是通过调节凋亡相关蛋白的表达。五味子多糖可能作为营养干预成分应用于功能性食品。
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