Department for Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
Department of Laboratory Diagnostics, Healthcare Centre Ada, Ada, Serbia.
Biochem Med (Zagreb). 2021 Feb 15;31(1):010709. doi: 10.11613/BM.2021.010709.
Indirect estimation of reference intervals (RIs) is straightforward and inexpensive procedure for determination of intra-laboratory RIs. We applied the indirect approach to assess RIs for haematological parameters in capillary blood of pre-school children, using results stored in our laboratory database.
We extracted data from laboratory information system, for the results obtained by automatic haematology analyser in capillary blood of 154 boys and 146 girls during pre-school medical examination. Data distribution was tested, and logarithmic transformation was applied if needed. Reference intervals were calculated by the nonparametric percentile method.
Reference intervals were calculated for: RBC count (4.2-5.4 x10/L), haemoglobin (114-146 g/L), MCH (25.0-29.4 pg), MCHC (321-368 g/L), RDW-SD (36.1-43.5 fL), WBC count (4.5-12.3 x10/L), neutrophils count (1.7-6.9 x10/L) and percentage (29.0-69.0%), lymphocytes count (1.6-4.4 x10/L) and percentage (21.9-60.7%), PLT (165-459 x10/L), MPV (8.1-11.4 fL) and PDW (9.2-14.4%). Gender specific RIs were calculated for monocytes count (male (M): 0.2-1.6 x10/L; female (F): 0.1-1.4 x10/L) and percentage (M: 2.5-18.3%; F: 1.8-16.7%), haematocrit (M: 0.34-0.42 L/L; F: 0.34-0.43 L/L), MCV (M: 73.4-84.6 fL; F: 75.5-84.2 fL) and RDW (M: 12.1-14.3%; F: 11.7-13.9%), due to observed gender differences in these parameters (P = 0.031, 0.028, 0.020, 0.012 and 0.001; respectively). Estimated RIs markedly varied from the literature based RIs that are used in the laboratory.
Indirect method employed in this study enables straightforward assessment of RIs in pre-school children. Herein derived RIs differed from the literature-based ones, indicating the need for intra-laboratory determination of RIs for specific populations and sample types.
间接估计参考区间（RI）是一种用于确定实验室内部 RI 的简单且经济的方法。我们应用间接方法来评估学龄前儿童毛细血管血液中血液学参数的 RI，使用存储在我们实验室数据库中的结果。
我们从实验室信息系统中提取数据，用于在学龄前体检期间通过自动血液分析仪获得的 154 名男孩和 146 名女孩的毛细血管血液的结果。测试了数据分布，如果需要，应用对数转换。通过非参数百分位数法计算参考区间。
计算了以下参数的参考区间：红细胞计数（4.2-5.4x10/L）、血红蛋白（114-146g/L）、MCH（25.0-29.4pg）、MCHC（321-368g/L）、RDW-SD（36.1-43.5fL）、白细胞计数（4.5-12.3x10/L）、中性粒细胞计数（1.7-6.9x10/L）和百分比（29.0-69.0%）、淋巴细胞计数（1.6-4.4x10/L）和百分比（21.9-60.7%）、血小板（165-459x10/L）、MPV（8.1-11.4fL）和 PDW（9.2-14.4%）。对于单核细胞计数（男性（M）：0.2-1.6x10/L；女性（F）：0.1-1.4x10/L）和百分比（M：2.5-18.3%；F：1.8-16.7%）和红细胞压积（M：0.34-0.42L/L；F：0.34-0.43L/L）、MCV（M：73.4-84.6fL；F：75.5-84.2fL）和 RDW（M：12.1-14.3%；F：11.7-13.9%），我们计算了性别特异性 RI，因为这些参数存在性别差异（P=0.031、0.028、0.020、0.012 和 0.001；分别）。估计的 RI 与实验室中使用的基于文献的 RI 有很大差异。
本研究中采用的间接方法可用于简单评估学龄前儿童的 RI。由此得出的 RI 与基于文献的 RI 不同，表明需要为特定人群和样本类型在实验室内部确定 RI。
