建立并验证中国南京地区 5-28 日龄新生儿的血液学参考区间。

Establishment and validation of haematological reference intervals for newborns aged 5 to 28 days in Nanjing, China.

机构信息

Department of Clinical Laboratory, Children's Hospital of Nanjing Medical University, Nanjing, People's Republic of China.

Department of Clinical Laboratory, Nanjing Maternity and Child Health Care Hospital, Nanjing, People's Republic of China.

出版信息

Hematology. 2023 Dec;28(1):2208891. doi: 10.1080/16078454.2023.2208891.

DOI:10.1080/16078454.2023.2208891

PMID:37183962

Abstract

BACKGROUND

China lacks a standard for reference intervals (RIs) of complete blood cell (CBC) counts in newborns. This study aimed to determine local haematological RIs for newborns.

METHODS

This prospective study was conducted from January 2020 to December 2020 in Nanjing Maternity and Child Health Care Hospital. We collected capillary blood specimens from 497 healthy newborns aged 5-28 days. We calculated the RIs as nonparametric 2.5th to 97.5th percentiles and 90% confidence intervals following the EP28-A3c guideline. We validated the RIs in another 20 specimens from healthy newborns.

RESULTS

The RIs for the 18 CBC parameters were: white blood cell count 7.17-15.69 × 109/L; monocytes# 0.52-1.66 × 109/L; Mono% 5.7-14.1; eosinophils# 0.16-1.08 × 109/L; Eos% 1.5-8.9; Lymphocytes# 4.04-8.08 × 109/L; Lymph% 40.1-67.8; Neutrophils# 1.59-6.41 × 109/L; Neut% 18.1-46.7; Basophils# 0.00-0.04 × 109/L; Baso% 0.0-0.4; red blood cell count 3.38-5.89 × 1012/L; haemoglobin (Hb) 116-198 g/L; Hematocrit % 35.2-61.2; mean corpuscular Hb (MCH) 30.9-36.3 pg/L; MCH concentration 310-341 g/L; mean corpuscular volume 94.5-112.2 fL; and platelets 210-610 × 109/L. The RIs had a conformity rate of 90%-100% in the validation specimens.

CONCLUSION

We established region-specific RIs for CBC parameters in healthy newborns to help diagnose haematological disease in neonates.

摘要

背景

中国缺乏新生儿全血细胞（CBC）计数参考区间（RI）的标准。本研究旨在确定新生儿局部血液学 RI。

方法

本前瞻性研究于 2020 年 1 月至 2020 年 12 月在南京市妇幼保健院进行。我们收集了 497 名 5-28 天龄健康新生儿的毛细血管血标本。我们按照 EP28-A3c 指南计算 RI 为非参数 2.5 至 97.5 百分位和 90%置信区间。我们在另外 20 名健康新生儿的标本中验证了 RI。

结果

18 个 CBC 参数的 RI 为：白细胞计数 7.17-15.69×109/L；单核细胞#0.52-1.66×109/L；单核细胞%5.7-14.1；嗜酸性粒细胞#0.16-1.08×109/L；嗜酸性粒细胞%1.5-8.9；淋巴细胞#4.04-8.08×109/L；淋巴细胞%40.1-67.8；中性粒细胞#1.59-6.41×109/L；中性粒细胞%18.1-46.7；嗜碱性粒细胞#0.00-0.04×109/L；嗜碱性粒细胞%0.0-0.4；红细胞计数 3.38-5.89×1012/L；血红蛋白（Hb）116-198g/L；血细胞比容%35.2-61.2；平均红细胞 Hb（MCH）30.9-36.3pg/L；MCH 浓度 310-341g/L；平均红细胞体积 94.5-112.2fL；血小板 210-610×109/L。验证标本的 RI 符合率为 90%-100%。