评估一种新型快速 TRC 检测法，使用鼻咽拭子和漱口样本检测流感。
Evaluation of a novel rapid TRC assay for the detection of influenza using nasopharyngeal swabs and gargle samples.
机构信息
Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, 852-8501, Japan.
Department of Laboratory Medicine, Nagasaki University Hospital, Nagasaki, Japan.
出版信息
Eur J Clin Microbiol Infect Dis. 2021 Aug;40(8):1743-1748. doi: 10.1007/s10096-021-04193-7. Epub 2021 Feb 16.
We evaluated a novel transcription-reverse transcription concerted reaction (TRC) assay that can detect influenza A and B within 15 min using nasopharyngeal swab and gargle samples obtained from patients with influenza-like illness, between January and March 2018 and between January and March 2019. Based on the combined RT-PCR and sequencing results, in the nasal swabs, the sensitivity and specificity of TRC for detecting influenza were calculated as 1.000 and 1.000, respectively. In the gargle samples, the sensitivity and specificity of TRC were 0.946 and 1.000, respectively. The TRC assay showed comparable performance to RT-PCR in the detection of influenza viruses.
我们评估了一种新型的转录-逆转录协同反应（TRC）检测方法，该方法可在 15 分钟内检测到流感 A 和 B，使用的是 2018 年 1 月至 3 月和 2019 年 1 月至 3 月期间流感样疾病患者的鼻咽拭子和漱口样本。基于联合 RT-PCR 和测序结果，在鼻拭子中，TRC 检测流感的灵敏度和特异性分别为 1.000 和 1.000。在漱口样本中，TRC 的灵敏度和特异性分别为 0.946 和 1.000。TRC 检测方法在流感病毒检测中的性能与 RT-PCR 相当。
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