McCarey B E, McNeill J I
Ann Ophthalmol. 1977 Oct;9(10):1279-83.
The corneal endothelium of intact isolated globes can be histologically evaluated with a specular microscope. The best observations are made within 6 to 8 postmortem hours; however, useful observations have been demonstrated up to 19 postmortem hours in a moist chamber stored eye at 4 C. The technique takes only a few minutes and enables one to see individual endothelial cell changes at magnifications of 120X to 400X. Endothelial screening can be done with this system for such changes as guttata, low cell populations, and trauma.
完整分离眼球的角膜内皮可通过镜面显微镜进行组织学评估。最佳观察时间是在死后6至8小时内；然而，在4℃潮湿环境中保存的眼球，在死后19小时内也能获得有用的观察结果。该技术只需几分钟，能让人在120倍至400倍的放大倍数下观察到单个内皮细胞的变化。利用该系统可进行内皮筛查，以检测诸如角膜小滴、低细胞数量和创伤等变化。
