Research and Development, Bio Supplies Division, Grifols, Barcelona, Spain.
R&D Department, Progenika Biopharma, A Grifols Company, Derio, Spain.
Diagn Microbiol Infect Dis. 2021 Jun;100(2):115340. doi: 10.1016/j.diagmicrobio.2021.115340. Epub 2021 Feb 6.
Seroconversion panels are an important tool for investigating antibody responses and developing serological assays. A seroconversion panel was generated from a single SARS-CoV-2 positive plasma donor over 87 days. This seroconversion panel was tested against 6 SARS-CoV-2 antibody tests (IgG, IgM, and total Ig). All test kits utilized recombinant antigens that are specific to SARS-CoV-2. The seroconversion panel showed IgG responses for SARS-CoV-2 after day 50. IgM levels peaked on day 50 (prior to IgG) and declined in subsequent samples. This seroconversion panel is a useful tool for validation of SARS-CoV-2 antibody assays.
血清转化panel 是研究抗体反应和开发血清学检测的重要工具。本研究从一位单一 SARS-CoV-2 阳性血浆捐献者中在 87 天内生成了血清转化 panel。该血清转化 panel 与 6 种 SARS-CoV-2 抗体检测试剂盒（IgG、IgM 和总 Ig）进行了测试。所有试剂盒均使用针对 SARS-CoV-2 的重组抗原。血清转化 panel 在第 50 天后出现了针对 SARS-CoV-2 的 IgG 反应。IgM 水平在第 50 天达到峰值（早于 IgG），随后在后续样本中下降。该血清转化 panel 是 SARS-CoV-2 抗体检测试剂盒验证的有用工具。
