Beijing Key Laboratory of DNA Damage Response and College of Life Sciences, Capital Normal University, Beijing, China.
Front Endocrinol (Lausanne). 2021 Feb 1;11:621888. doi: 10.3389/fendo.2020.621888. eCollection 2020.
The centrosome apparatus is vital for spindle assembly and chromosome segregation during mitotic divisions. Its replication, disjunction and separation have to be fine-tuned in space and time. A multitude of post-translational modifications (PTMs) have been implicated in centrosome modulation, including phosphorylation, ubiquitination and acetylation. Among them is the emerging O-linked N-acetylglucosamine (O-GlcNAc) modification. This quintessential PTM has a sole writer, O-GlcNAc transferase (OGT), and the only eraser, O-GlcNAcase (OGA). O-GlcNAc couples glucose metabolism with signal transduction and forms a yin-yang relationship with phosphorylation. Evidence from proteomic studies as well as single protein investigations has pinpointed a role of O-GlcNAc in centrosome number and separation, centriole number and distribution, as well as the cilia machinery emanating from the centrosomes. Herein we review our current understanding of the sweet modification embedded in centrosome dynamics and speculate that more molecular details will be unveiled in the future.
中心体装置对于有丝分裂过程中纺锤体的组装和染色体的分离至关重要。其复制、分离和分离必须在空间和时间上进行精细调整。大量的翻译后修饰（PTMs）被牵涉到中心体的调节中，包括磷酸化、泛素化和乙酰化。其中新兴的 O-连接 N-乙酰葡萄糖胺（O-GlcNAc）修饰。这种典型的 PTM 只有一个作者，O-连接 N-乙酰葡萄糖胺转移酶（OGT），和唯一的橡皮擦，O-连接 N-乙酰氨基葡萄糖苷酶（OGA）。O-GlcNAc 将葡萄糖代谢与信号转导偶联，与磷酸化形成阴阳关系。蛋白质组学研究以及单一蛋白质研究的证据指出，O-GlcNAc 在中心体数量和分离、中心粒数量和分布以及从中心体发出的纤毛机制中起作用。本文综述了我们目前对嵌入中心体动力学的甜蜜修饰的理解，并推测未来将揭示更多的分子细节。
