Bioingénierie et Dynamique Microbienne aux Interfaces Alimentaires (BioDyMIA, EA n°3733), IUT Lyon 1 site de Bourg en Bresse - Technopole Alimentec, Université de Lyon, Université Claude Bernard, Lyon 1 - ISARA Lyon, rue Henri de Boissieu, Bourg en Bresse, France.
Ecole de Biotechnologie et Technologie Alimentaire, Institut Polytechnique de Hanoi, Hanoi, Vietnam.
Lett Appl Microbiol. 2021 Jun;72(6):757-766. doi: 10.1111/lam.13465. Epub 2021 Mar 23.
The antibacterial activity of a Cinnamomum cassia essential oil (EO) and of its main component trans-cinnamaldehyde (90% w/w) was examined against five Listeria monocytogenes strains. The minimal inhibitory concentrations (MICs) of C. cassia EO against the five L. monocytogenes strains were identical (250 µg ml ), while the minimal bactericidal concentrations (MBCs) ranged between 800 and 1200 µg ml . In order to study if this EO and trans-cinnamaldehyde altered the five strains at the membrane level, fluorescence anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH) was measured in presence of different concentrations (1/2MIC, MIC, 2MIC) of these antibacterial agents. A concentration-dependent increase of fluorescence anisotropy of DPH in their presence reflecting a rigidification of the membrane was observed for the five strains. This modification of the membrane fluidity was associated with a perturbation of the selective membrane permeability, as a perturbation of the gradient between intracellular and extracellular pH was also observed.
肉桂精油（EO）及其主要成分反式肉桂醛（90%w/w）对 5 株单核细胞增生李斯特菌的抗菌活性进行了研究。肉桂精油（EO）对 5 株单核细胞增生李斯特菌的最小抑菌浓度（MIC）相同（250μg/ml），而最小杀菌浓度（MBC）在 800 至 1200μg/ml 之间。为了研究这种精油和反式肉桂醛是否会改变 5 株菌株的膜水平，在不同浓度（1/2MIC、MIC、2MIC）的这些抗菌剂存在下，测量了 1,6-二苯基-1,3,5-己三烯（DPH）的荧光各向异性。在这些抗菌剂的存在下，DPH 的荧光各向异性呈浓度依赖性增加，反映出膜的刚性增加，这种膜流动性的改变与选择性膜通透性的扰动有关，因为还观察到细胞内和细胞外 pH 值梯度的扰动。
