Giordani R, Regli P, Kaloustian J, Portugal H
Laboratoire de Botanique, Cryptogamie et Biologie Cellulaire, Faculté de Pharmacie, Université de la Méditerranée, 27 Bd Jean Moulin, 13385 Marseille Cedex 05, France.
Phytother Res. 2006 Jan;20(1):58-61. doi: 10.1002/ptr.1803.
The antifungal activity of the essential oil from Cinnamomum cassia, alone or combined with amphotericin B, a drug widely used for most indications despite side-effects was investigated. The composition of the oil was analysed by GC/MS and characterized by its very high content of cinnamaldehyde (92.2%). The minimal inhibitory concentration (MIC 80%), used to evaluate the antifungal activity against Candida albicans, was determined by a macrobroth dilution method followed by a modelling of fungal growth. The essential oil of Cinnamomum cassia exhibited strong antifungal effect (MIC 80% = 0.169 microL/mL and K(aff) = 18,544 microL/mL). A decrease of the MIC 80% of amphotericin B was obtained when the culture medium contained essential oil concentrations ranging from 0.08 to 0.1 microL/mL. The strongest decrease (70%) was obtained when the medium contained 0.1 microL/mL of essential oil. This potentiation of amphotericin B obtained in vitro may show promise for the development of less toxic and more effective therapies especially for the treatment of HIV infection.
研究了肉桂精油单独或与两性霉素B联合使用时的抗真菌活性。两性霉素B是一种尽管有副作用但仍广泛用于大多数适应症的药物。通过气相色谱/质谱联用仪（GC/MS）分析了该精油的成分，其特征在于肉桂醛含量非常高（92.2%）。采用常量肉汤稀释法并对真菌生长进行建模，测定了用于评估对白色念珠菌抗真菌活性的最低抑菌浓度（MIC 80%）。肉桂精油表现出很强的抗真菌作用（MIC 80% = 0.169微升/毫升，亲和常数K(aff) = 18544微升/毫升）。当培养基中精油浓度在0.08至0.1微升/毫升范围内时，两性霉素B的MIC 80%降低。当培养基中含有0.1微升/毫升精油时，降幅最大（70%）。体外获得的两性霉素B这种增效作用可能为开发毒性更小、更有效的疗法带来希望，尤其是用于治疗艾滋病毒感染。
