Department of Emergency, Xi'an Children's Hospital, The Affiliated Children's Hospital of Xi'an Jiaotong University), Xi'an, Shaanxi, P.R. China.
School of Public Health, Xi'an Jiaotong University, Xi'an, Shaanxi, P.R. China.
Brain Behav. 2021 Apr;11(4):e02046. doi: 10.1002/brb3.2046. Epub 2021 Feb 18.
To explore the changes in C-reactive protein (CRP) level in different regions of one old offspring rats exposed to prenatal stress (PS).
The rat model was constructed with prenatal restraint stress on pregnant dams on days 14-20 of gestation. Offspring rats were randomly divided into PS susceptibility (PS-S) group and control (CON) group. Behavioral experiments including sucrose preference test (SPT), open-field test (OFT), and forced swimming test (FST) were used to measure depressive-like behaviors. Immunohistochemistry, qRT-PCR, and Western blotting were applied to detect the changes in CRP level.
The results showed that PS could cause depressive-like behaviors in all SPT, OFT, and FST. Concomitantly, CRP mRNA and protein expression significantly increased in hippocampus, prefrontal cortex, and hypothalamus in the PS-S group when compared that in the CON group, while no significantly changes in liver, heart, olfactory bulb, striatum, and cerebellum in the PS-S group when compared that in the CON group.
Increasing of CRP expression in hippocampus, prefrontal cortex, and hypothalamus may play a critical role in the mechanism under depressive-like behavior in offspring rats exposed to PS.
探讨产前应激（PS）暴露的老年后代大鼠不同区域 C 反应蛋白（CRP）水平的变化。
在妊娠第 14-20 天对孕鼠进行产前束缚应激，构建大鼠模型。后代大鼠随机分为 PS 易感性（PS-S）组和对照组（CON）。采用糖水偏好试验（SPT）、旷场试验（OFT）和强迫游泳试验（FST）等行为学实验检测抑郁样行为。免疫组织化学、qRT-PCR 和 Western blot 用于检测 CRP 水平的变化。
结果表明，PS 可导致 SPT、OFT 和 FST 中所有大鼠出现抑郁样行为。同时，PS-S 组大鼠海马体、前额叶皮层和下丘脑的 CRP mRNA 和蛋白表达显著增加，而 PS-S 组大鼠肝脏、心脏、嗅球、纹状体和小脑的 CRP 表达无显著变化。
在 PS 暴露的后代大鼠的抑郁样行为机制中，海马体、前额叶皮层和下丘脑 CRP 表达的增加可能起关键作用。
