Garris D R
Department of Anatomy, Cleveland Chiropractic College Research Laboratory, Kansas City, Missouri 64131.
Endocrinology. 1988 May;122(5):2183-90. doi: 10.1210/endo-122-5-2183.
The ability of ovarian steroid hormones to modulate experimentally induced decidual tissue (DT) growth and the associated changes in uterine blood flow rates (UBF) was examined in ovariectomized guinea pigs after uterine trauma (designated day 0 of the studies). Uteri that were exposed, but not manipulated, served as controls. Uterine and DT weights as well as UBF, rates, were subsequently recorded on either day 5 or 10 posttrauma. Oil treatment failed to induce an increase in either control or traumatized uterine weights between days 5 and 10, and trauma had no effect on UBF rates in either group. Daily progesterone (P; 2 mg) treatment induced a significant elevation in DT weight by day 10 and elevated UBF rates between days 5 and 10 relative to control values. Daily P treatment augmented by estradiol (E2; 1 microgram) therapy on days 0 and 1 induced a significant increase in DT weights and UBF rates between days 5 and 10 in both control and DT groups relative to those in oil-treated animals. Combined P and E2 (P/E2) treatment induced a moderate increase in DT weight by day 10 posttrauma and elevated UBF rates in both control and DT groups. Acute treatment (i.e. days -3 to 0) with these steroid regimens indicated that neither P nor P/E2 treatment maintained DT growth. However, day -3 to 0 treatment with P in combination with a single day 0 injection of E2 allowed for maximal DT growth by day 10 and maintained elevated UBF rates relative to control values. P/E2 treatment between days -3 and 0 also induced an increase in UBF rates in both control and DT uteri relative to those in oil-treated animals. These results indicate that E2 is essential for supporting the P-directed differentiation and proliferation of stimulated guinea pig endometrium into DT. The ability of decidualization to occur in the absence of chronic steroid support indicates that uterine sensitization for cellular differentiation in this species only requires that the endometrium be initially primed by ovarian steroid hormones, but that subsequent growth is autonomous.
在子宫创伤后（研究指定的第0天），对卵巢切除的豚鼠进行实验，以研究卵巢甾体激素调节实验性诱导的蜕膜组织（DT）生长以及子宫血流速率（UBF）相关变化的能力。暴露但未进行操作的子宫作为对照。随后在创伤后第5天或第10天记录子宫和DT重量以及UBF速率。在第5天至第10天期间，油处理未能使对照或创伤子宫重量增加，且创伤对两组的UBF速率均无影响。每日注射孕酮（P；2mg）至第10天时，DT重量显著升高，且在第5天至第10天期间UBF速率相对于对照值升高。在第0天和第1天，每日P处理联合雌二醇（E₂；1μg）治疗，相对于油处理动物，在第5天至第10天期间，对照和DT组的DT重量和UBF速率均显著增加。联合P和E₂（P/E₂）处理在创伤后第10天时使DT重量适度增加，且对照和DT组的UBF速率均升高。用这些甾体激素方案进行急性处理（即第 - 3天至第0天）表明，单独的P或P/E₂处理均不能维持DT生长。然而，第 - 3天至第0天用P处理并在第0天单次注射E₂，到第10天时可使DT最大程度生长，且相对于对照值维持UBF速率升高。第 - 3天至第0天进行P/E₂处理，相对于油处理动物，对照和DT子宫的UBF速率也增加。这些结果表明，E₂对于支持P引导的受刺激豚鼠子宫内膜向DT的分化和增殖至关重要（重要性）。在缺乏慢性甾体激素支持的情况下发生蜕膜化表明，该物种子宫对细胞分化的致敏仅要求子宫内膜最初由卵巢甾体激素启动，但随后的生长是自主的。
