Politis M J
Department of Anatomy, School of Medicine, University of Saskatchewan, Saskatoon, Canada.
Exp Neurol. 1988 May;100(2):288-96. doi: 10.1016/0014-4886(88)90108-2.
Exogenous myelin- or axolemma-enriched fractions were assessed for the ability to inhibit biochemical and morphological expressions of reactive gliosis in rat optic nerve. Elvax pellets containing exogenous myelin, axolemma, whole-brain homogenate, liver, or red cell extracts or no homogenate were inserted into a dural slit in distal regions of crushed optic nerve. Biochemical and morphological expressions of reactive gliosis were assessed at 7 or 14 days postoperatively. Post-traumatic elevations in lactic dehydrogenase activity normally seen at 7 days postoperatively were prevented by placement of Elvax pellets containing myelin or axolemmal fractions into the optic nerve. Morphological analyses indicated an inhibition of post-traumatic elevations in glial cell numbers, surface area, and nuclear size at the 14-day time point. Exposure of the axolemmal fraction to heat or trypsin inactivated its ability to modulate reactive gliotic changes. Myelin fractions were trypsin-sensitive, but not heat-sensitive. In contrast, Elvax pellets containing whole-brain tissue homogenates or liver and red cell membranes had no significant effects on post-traumatic glial changes relative to preparations in which homogenate-free pellets were used.
评估外源性富含髓磷脂或轴膜的组分抑制大鼠视神经中反应性胶质增生的生化和形态学表现的能力。将含有外源性髓磷脂、轴膜、全脑组织匀浆、肝脏或红细胞提取物或不含匀浆的乙烯-醋酸乙烯共聚物（Elvax）微丸插入视神经挤压远端区域的硬脑膜裂隙中。在术后7天或14天评估反应性胶质增生的生化和形态学表现。术后7天通常出现的创伤后乳酸脱氢酶活性升高通过将含有髓磷脂或轴膜组分的Elvax微丸植入视神经得以预防。形态学分析表明在14天时间点创伤后胶质细胞数量、表面积和核大小的增加受到抑制。轴膜组分经加热或胰蛋白酶处理后会使其调节反应性胶质变化的能力丧失。髓磷脂组分对胰蛋白酶敏感，但对热不敏感。相比之下，相对于使用不含匀浆微丸的制剂，含有全脑组织匀浆或肝脏及红细胞膜的Elvax微丸对创伤后胶质变化无显著影响。
