Quiot J M
Arch Virol. 1977;55(1-2):145-53. doi: 10.1007/BF01314488.
A virus isolated from the larvaes of Euxoa scandens (Noctuidae, Agrotinae) was studied by histological and histochemical techniques, and by electron microscopic observation of virus suspensions and ultrathin sections of infected tissues. Our studies demonstrated that the virus in icosahedral in shape, measures 70 nm and belongs to the cytoplasmic polyhedrosis virus group. Only the midgut cells of the larvae were found to be infected and the virus particles were localized in inclusion bodies most of which had a paraspherical, polyhedral shape. It was possible to infect Lymantria dispar L. cells in vitro with free virus or with extracts obtained from the diseased larvae. The virogenic stroma and the first small inclusion bodies in this stroma were detected in the cytoplasm at 18 and 36 hours post infection, respectively. These polyhedra had a cubical shape, reached their maximum size after 48 hours and were located in the cytoplasm of over 90 percent of the cells.
利用组织学和组织化学技术以及对病毒悬液和受感染组织超薄切片的电子显微镜观察，对从斯堪的纳维亚夜蛾（夜蛾科，切根虫亚科）幼虫中分离出的一种病毒进行了研究。我们的研究表明，该病毒呈二十面体形状，直径为70纳米，属于细胞质多角体病毒组。仅发现幼虫的中肠细胞受到感染，病毒粒子位于包涵体内，其中大多数呈近球形、多面体形状。用游离病毒或从患病幼虫中获得的提取物在体外感染舞毒蛾细胞是可行的。分别在感染后18小时和36小时在细胞质中检测到病毒发生基质和该基质中的第一批小包涵体。这些多面体呈立方体形状，在48小时后达到最大尺寸，并位于90%以上细胞的细胞质中。
