Milner R J, Beaton C D
Intervirology. 1979;11(6):341-50. doi: 10.1159/000149056.
A new entomopoxvirus has been isolated from field-collected larvae of Oncopera alboguttata at Ebor, New South Wales, Australia. The ovoid proteinaceous virus-containing bodies measured about 8 x 6 microns and contained up to about 17 centrally-embedded virus-free spindles and up to about 180 occluded virus particles. The virus particle had a beaded outer membrane and measured about 390 x 270 x 230 nm. The particle contained a single lateral body and a unilaterally concave core which in longitudinal section measured 320 x 80 nm. Dissolution of inclusion body and spindle protein to release virus particles occurred in carbonate/thioglycollate buffer at pH greater than 12.0. It is postulated that this virus has recently evolved from similar viruses in soil inhabiting coleopteran larvae.
一种新的昆虫痘病毒已从澳大利亚新南威尔士州埃博尔野外采集的白斑澳夜蛾幼虫中分离出来。卵形含蛋白质的病毒包涵体大小约为8×6微米,含有多达约17个位于中央的无病毒纺锤体和约180个被包埋的病毒粒子。病毒粒子有带珠状的外膜,大小约为390×270×230纳米。该粒子包含一个侧体和一个单侧凹陷的核心,在纵切面上大小为320×80纳米。在pH值大于12.0的碳酸盐/巯基乙酸盐缓冲液中,包涵体和纺锤体蛋白溶解以释放病毒粒子。据推测,这种病毒最近从栖息在土壤中的鞘翅目幼虫的类似病毒进化而来。