Bhoite Roopali, Si Ping, Siddique Kadambot H M, Yan Guijun
UWA School of Agriculture and Environment, The University of Western Australia, Perth, WA 6009, Australia; The UWA Institute of Agriculture, The University of Western Australia, Perth, WA 6009, Australia.
The UWA Institute of Agriculture, The University of Western Australia, Perth, WA 6009, Australia.
Genomics. 2021 May;113(3):910-918. doi: 10.1016/j.ygeno.2021.02.004. Epub 2021 Feb 16.
Weeds are the biggest threat to cropping system sustainability in wheat. Metribuzin is a versatile herbicide for broad-spectrum weed management. Understanding key genes, mechanisms and functional markers are essential to develop higher metribuzin tolerant wheats. We identified Chuan Mai 25 (tolerant) and Ritchie (susceptible) as contrasting genotypes to metribuzin stress through dose-response analyses. Transcriptome sequencing using NovaSeq 6000 RNA-Seq platform identified a total of 77,443 genes; 59,915 known genes and 17,528 novel genes. The functional enrichment analysis at 0 h, 24 h and 60 h herbicide exposure revealed that endogenous increase of metabolic enzymes, light-harvesting chlorophyll proteins, PSII stability factor HCF136 and glucose metabolism conferred metribuzin tolerance. The validation of DEGs using RT-qPCR and QTL mapping confirmed their responsiveness to metribuzin. Transcription factors MYB, AP2-EREBP, ABI3VP1, bHLH, NAC are significantly expressed during metribuzin stress. Transcripts with significant enrichments revealed 114 SSRs for genomic selection. The master regulators provide promising avenues for enhancing metribuzin tolerance.
杂草是小麦种植系统可持续性的最大威胁。嗪草酮是一种用于广谱杂草治理的多功能除草剂。了解关键基因、机制和功能标记对于培育更高嗪草酮耐受性的小麦至关重要。通过剂量反应分析，我们确定川麦25（耐受型）和里奇（敏感型）为对嗪草酮胁迫具有对比性的基因型。使用NovaSeq 6000 RNA-Seq平台进行转录组测序共鉴定出77443个基因；其中59915个已知基因和17528个新基因。在除草剂处理0小时、24小时和60小时时进行的功能富集分析表明，代谢酶、捕光叶绿素蛋白、PSII稳定性因子HCF136和葡萄糖代谢的内源性增加赋予了对嗪草酮的耐受性。使用RT-qPCR对差异表达基因进行验证和QTL定位证实了它们对嗪草酮的反应。转录因子MYB、AP2-EREBP、ABI3VP1、bHLH、NAC在嗪草酮胁迫期间显著表达。具有显著富集的转录本揭示了114个用于基因组选择的SSR。这些主要调控因子为提高嗪草酮耐受性提供了有前景的途径。
