近等基因系转录组分析揭示了赋予小麦对苯达嗪抗性的显著位点的候选基因。

Transcriptomic profiling of near-isogenic lines reveals candidate genes for a significant locus conferring metribuzin resistance in wheat.

机构信息

UWA School of Agriculture and Environment, The University of Western Australia, 6009, Perth, WA, Australia.

The UWA Institute of Agriculture, The University of Western Australia, 6009, Perth, WA, Australia.

出版信息

BMC Plant Biol. 2023 May 5;23(1):237. doi: 10.1186/s12870-023-04166-2.

DOI:10.1186/s12870-023-04166-2

PMID:37142987

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10161546/

Abstract

BACKGROUND

Weeds reduce wheat yields in dryland farming systems. Herbicides such as metribuzin are commonly used to control weeds. However, wheat has a narrow safety margin against metribuzin. Standing crops such as wheat with weeds in the same field can also be killed by the same dose of metribuzin. Therefore, it is important to identify metribuzin resistance genes and understand the resistance mechanism in wheat for sustainable crop production. A previous study identified a significant metribuzin resistance wheat QTL, Qsns.uwa.4 A.2, explaining 69% of the phenotypic variance for metribuzin resistance.

RESULTS

Two NIL pairs with the most contrasting performance in the metribuzin treatment and different in genetic backgrounds were compared using RNA sequence analysis, identifying nine candidate genes underlying Qsns.uwa.4 A.2 responsible for metribuzin resistance. Quantitative RT-qPCR further validated the candidate genes, with TraesCS4A03G1099000 (nitrate excretion transporter), TraesCS4A03G1181300 (aspartyl protease), and TraesCS4A03G0741300 (glycine-rich proteins) identified as key factors for metribuzin resistance.

CONCLUSION

Identified markers and key candidate genes can be used for selecting metribuzin resistance in wheat.

摘要

背景

杂草会降低旱地农业系统中的小麦产量。二甲戊灵等除草剂常用于控制杂草。然而，小麦对二甲戊灵的安全边际很窄。同一田块中既有小麦又有杂草时，同样剂量的二甲戊灵也会杀死作物。因此，对于可持续的作物生产，识别小麦中的二甲戊灵抗性基因并了解其抗性机制非常重要。先前的研究确定了一个显著的抗二甲戊灵小麦 QTL，Qsns.uwa.4A.2，解释了 69%的二甲戊灵抗性表型变异。

结果

使用 RNA 序列分析比较了在二甲戊灵处理中表现出最显著差异且遗传背景不同的两个近等基因系对，确定了九个候选基因，这些基因是导致 Qsns.uwa.4A.2 产生抗二甲戊灵的原因。定量 RT-qPCR 进一步验证了候选基因，其中 TraesCS4A03G1099000（硝酸盐排泄转运蛋白）、TraesCS4A03G1181300（天冬氨酸蛋白酶）和 TraesCS4A03G0741300（甘氨酸丰富蛋白）被鉴定为抗二甲戊灵的关键因素。