Miura T, Komori M, Iwasaki M, Kurozumi K, Ohta K, Ohmori S, Kitada M, Kamataki T
Division of Analytical Biochemistry, Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo, Japan.
FEBS Lett. 1988 Apr 11;231(1):183-6. doi: 10.1016/0014-5793(88)80727-0.
The activities of testosterone hydroxylases and erythromycin N-demethylase were significantly higher in liver microsomes from female hamsters than in the male counterparts. SDS-polyacrylamide gel electrophoresis revealed a difference in protein composition between male and female liver microsomes in the molecular mass region comprising cytochrome P-450. Western blot analysis showed further that antibodies to rat male-specific cytochrome P-450 crossreacted with at least two proteins in both male and female hamster microsomes, but one of the female proteins had a different molecular mass from that of the male proteins. It is concluded that sex difference in liver microsomal cytochrome P-450 is not restricted to rats and mice, as has previously been believed.
雌性仓鼠肝脏微粒体中睾酮羟化酶和红霉素N-脱甲基酶的活性显著高于雄性仓鼠的相应酶活性。SDS-聚丙烯酰胺凝胶电泳显示，在包含细胞色素P-450的分子量区域内，雄性和雌性肝脏微粒体的蛋白质组成存在差异。蛋白质印迹分析进一步表明，针对大鼠雄性特异性细胞色素P-450的抗体与雄性和雌性仓鼠微粒体中的至少两种蛋白质发生交叉反应，但雌性的一种蛋白质与雄性蛋白质的分子量不同。得出的结论是，肝脏微粒体细胞色素P-450的性别差异并不像以前所认为的那样仅限于大鼠和小鼠。
