Schoofs L, Schrooten S, Huybrechts R, De Loof A
Zoological Institute of the University, Leuven, Belgium.
Gen Comp Endocrinol. 1988 Jan;69(1):1-12. doi: 10.1016/0016-6480(88)90046-9.
Methionine(met)-enkephalin immunoreactivity as visualized by the peroxidase-antiperoxidase procedure, is present in spermatogonia, spermatocytes, spermatids, and young ovarian follicles of Locusta (panoistic type) and Sarcophaga (polytrophic type). Follicle cells and mature spermatozoa are always immunonegative as are locust vitellogenic follicles. In oocytes and in trophocytes, the met-enkephalin-like material first appears around the nucleus and is then dispersed throughout the cytoplasm. Later, it is present only in the periphery. In the ovary of both insects, no immunoreactivity is found with antisera against adrenocorticotrophic hormone, melanophore stimulating hormone, beta-endorphin, corticotropin releasing factor, or leucine-enkephalin. All these antisera yield a positive reaction when applied to the central nervous system as does the met-enkephalin antiserum. This study indicates that the met-enkephalin-like peptide may play a role in reproductive physiology.
通过过氧化物酶-抗过氧化物酶法观察到的甲硫氨酸（Met）-脑啡肽免疫反应性，存在于飞蝗（无滋式类型）和肉蝇（多滋式类型）的精原细胞、精母细胞、精子细胞以及年轻的卵巢卵泡中。卵泡细胞和成熟精子始终呈免疫阴性，飞蝗的卵黄生成卵泡也是如此。在卵母细胞和滋养细胞中，Met-脑啡肽样物质首先出现在细胞核周围，然后散布于整个细胞质中。之后，它仅存在于周边区域。在这两种昆虫的卵巢中，针对促肾上腺皮质激素、促黑素细胞激素、β-内啡肽、促肾上腺皮质激素释放因子或亮氨酸-脑啡肽的抗血清均未发现免疫反应性。当将所有这些抗血清应用于中枢神经系统时，与Met-脑啡肽抗血清一样都会产生阳性反应。这项研究表明，Met-脑啡肽样肽可能在生殖生理学中发挥作用。
