大脑中催乳素结合位点的区域分布及物种差异

Regional distribution and species variation of prolactin binding sites in the brain.

作者信息

Muccioli G, Bellussi G, Ghé C, Pagnini G, Di Carlo R

机构信息

Institute of Pharmacology, University of Turin, Italy.

出版信息

Gen Comp Endocrinol. 1988 Mar;69(3):399-405. doi: 10.1016/0016-6480(88)90031-7.

DOI:10.1016/0016-6480(88)90031-7

PMID:3360296

Abstract

The binding of 125I-labeled ovine prolactin (125I-oPRL) to membranes from different brain regions of pigeon, rabbit, rat, pig, calf, horse, and ewe was studied. The hypothalamus from rabbit, pig, horse, and pigeon showed a low but specific binding for 125I-oPRL clearly different from the other brain regions examined (cortex and cerebellum), whereas in the brain from rat, calf, and ewe the binding was very small and quite uniform in the various regions. Also the membranes from choroid plexus of rabbit, pig, calf, and horse showed an evident specific binding for prolactin. The binding of 125I-oPRL to hypothalamus and choroid plexus membranes from rabbit and horse was inhibited in a dose-dependent manner by unlabeled oPRL and hGH but not by many other polypeptide hormones. Scatchard analysis of the binding revealed the presence of binding sites with low capacity and high affinity for ovine ligand.

摘要

研究了125I标记的绵羊催乳素（125I-oPRL）与鸽、兔、大鼠、猪、小牛、马和母羊不同脑区膜的结合情况。兔、猪、马和鸽的下丘脑对125I-oPRL显示出低但特异性的结合，明显不同于所检查的其他脑区（皮层和小脑），而在大鼠、小牛和母羊的脑中，结合非常少且在各个区域相当均匀。兔、猪、小牛和马的脉络丛膜对催乳素也显示出明显的特异性结合。未标记的oPRL和hGH以剂量依赖的方式抑制125I-oPRL与兔和马的下丘脑及脉络丛膜的结合，但许多其他多肽激素则无此作用。对结合的Scatchard分析表明存在对绵羊配体具有低容量和高亲和力的结合位点。