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柬埔寨水稻(L.)种质的遗传变异

Genetic variation of rice ( L.) germplasm in Cambodia.

作者信息

Orn Chhourn, Saito Hiroki, Khan Mohammad Ashik Iqbal, Nhuiyan Mohammad Rajiwan, Vathany Thun, Khay Sathaya, Makara Ouk, Fukuta Yoshimichi

机构信息

Plant Breeding Division, Cambodian Agricultural Research and Development Institute (CARDI), Phnom Penh, Cambodia.

Tropical Agriculture Research Front (TARF), Japan International Research Center for Agricultural Sciences (JIRCAS), 1019-1 Kawarabaru, Ishigaki, Okinawa 907-0002, Japan.

出版信息

Breed Sci. 2020 Dec;70(5):576-585. doi: 10.1270/jsbbs.20052. Epub 2020 Nov 28.

Abstract

Genetic variations of 179 rice ( L.) accessions from Cambodia were clarified based on the analyses for heading date, chromosome components, and blast resistance. The dominant accessions were found in three regions; early heading in North East (NE), medium in Central (CT), and late in South East (SE) along the Mekong River in the investigation at Ishigaki, Japan. In contrast, wide variations were observed in two regions, South West (SW) and North West (NW) located around Tonle Sap Lake. Polymorphism data of SSR markers showed that accessions were classified into Japonica Group (cluster Ib), and Indica Groups (IIa and IIb). In the NW and SW, the accessions of all three clusters were found, but these accessions in NE, CT, and SE, were limited to one or two clusters. Accessions were classified again into two clusters, A1 as having high resistance and A2 as having moderate resistance. Remarkable differences of these frequencies of clusters, A1 and A2, were found in the SE, SW, and NW, and similar with these of the whole accessions were in NE and CT. Rice accessions varied among the five regions, and there was a dramatic difference between the regions along Mekong River and the regions around Tonle Sap Lake.

摘要

基于抽穗期、染色体组成和稻瘟病抗性分析,明确了来自柬埔寨的179份水稻种质的遗传变异。在日本石垣岛的调查中,优势种质分布在三个区域:湄公河沿岸东北部(NE)抽穗早、中部(CT)抽穗适中、东南部(SE)抽穗晚。相比之下,在洞里萨湖周边的西南(SW)和西北(NW)两个区域观察到了广泛的变异。SSR标记的多态性数据表明,种质被分为粳稻组(聚类Ib)和籼稻组(IIa和IIb)。在NW和SW发现了所有三个聚类的种质,但NE、CT和SE的这些种质仅限于一两个聚类。种质再次被分为两个聚类,A1为高抗性,A2为中等抗性。在SE、SW和NW发现了聚类A1和A2频率的显著差异,而NE和CT与所有种质的频率相似。水稻种质在五个区域间存在差异,湄公河沿岸地区和洞里萨湖周边地区之间存在显著差异。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/38d6/7878939/6967d38eab31/70_576-g001.jpg

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