Proteomic analysis of in response to different pHs using two-dimensional electrophoresis and MALDI TOF mass spectroscopy.

BACKGROUND AND OBJECTIVES

, an acid-resistant bacterium, has a protective role against the pathogens. So we aimed to determine the proteome of ATCC39392 strain in response to different pHs of 5 and 7 using proteomic analysis.

MATERIALS AND METHODS

Supernatant and bacterial extraction of ATCC39392 adapts at pHs 5 and 7 were isolated using sodium dodecyl sulfate-polyacrylamide gel and two-dimensional gel electrophoresis. The comparison of results showed that 7 protein spots were seen in pH 5 but not in pH 7. Afterward, they were excised and sent for Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) to be identified.

RESULTS

Seven different proteins (four secretory and three structural) with different roles in human body health were identified. Prescribed proteins include putative cell wall associated Hydrolase, Glycoside Hydrolase, beta-N-Acetyl hexosaminidase, Histidine Kinase, Chaperonin, metal dependent Hydrolase and Lysozyme.

CONCLUSION

Seven isolated proteins with anti-cancer and digestive impresses are proper subjects in therapy or drug delivery approaches especially oral drug usage for protection against stomach acidic area.