Hebda Anna, Kempf Marta, Wachowiak Witold, Pluciński Bartosz, Kauzal Paweł, Zwijacz-Kozica Tomasz
Department of Plant Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University in Kraków, Kraków, Poland.
Department of Genetics and Forest Tree Breeding, Institute of Forest Ecology and Silviculture, Faculty of Forestry, University of Agriculture in Krakow, Kraków, Poland.
AoB Plants. 2020 Dec 30;13(1):plaa070. doi: 10.1093/aobpla/plaa070. eCollection 2021 Feb.
Hybridization and introgression are important processes influencing the genetic diversity and evolution of species. These processes are of particular importance in protected areas, where they can lead to the formation of hybrids between native and foreign species and may ultimately result in the loss of parental species from their natural range. Despite their importance, the contribution of hybridization and introgression to genetic diversity in genus remains not fully recognized. We analysed the genetic and morphological variability of several species including native (), foreign () and potentially hybrid () individuals from the Polish Carpathian range. Patterns of variation at 13 nuclear microsatellite loci show hybridization between the tested species and confirm the existence of the hybrid form . Biometric analysis on leaves, based of 10 metric features and three parameters, identified several characters for preliminary taxonomic classification; however, none of them could be used as a fully diagnostic marker for faultless annotation of and . The genetic structure analysis indicated complex patterns of population differentiation and its diverse origin. The results allow assessment of genetic variation and identification of parental species participating in hybridization. This knowledge will advance the management of genetic diversity and development of conservation strategies for efficient maintenance of the unique protected ecosystem.
杂交和基因渗入是影响物种遗传多样性和进化的重要过程。这些过程在保护区尤为重要，因为它们可能导致本地物种与外来物种之间形成杂交种，并最终可能导致亲本物种在其自然分布范围内消失。尽管杂交和基因渗入很重要，但它们对该属遗传多样性的贡献仍未得到充分认识。我们分析了几个物种的遗传和形态变异性，包括来自波兰喀尔巴阡山脉地区的本地（）、外来（）和潜在杂交（）个体。13个核微卫星位点的变异模式显示了受试物种之间的杂交，并证实了杂交形式的存在。基于10个度量特征和3个参数对叶片进行的生物统计分析，确定了几个用于初步分类的特征；然而，它们都不能用作完全诊断性标记来无误地鉴定和。遗传结构分析表明了种群分化的复杂模式及其多样的起源。这些结果有助于评估遗传变异，并识别参与杂交的亲本物种。这些知识将推动遗传多样性管理和保护策略的制定，以有效地维护独特的受保护生态系统。
