Mendler-Drienyovszki Nóra, Magyar-Tábori Katalin
Research Institute of Nyíregyháza, Institutes for Agricultural Research and Educational Farm (IAREF), University of Debrecen, P.O. Box 12, 4400 Nyiregyhaza, Hungary.
Plants (Basel). 2023 Mar 12;12(6):1287. doi: 10.3390/plants12061287.
Slow growth storage can preserve the genetic resources of endangered species such as those of genus . Our aim was to study the storability of rowan berry in vitro cultures, their morpho-physiological changes, and regeneration ability after different storage conditions (4 ± 0.5 °C, dark; and 22 ± 2 °C, 16/8 h light/dark). The cold storage lasted for 52 weeks, and observations were made every four weeks. Cultures showed 100% survival under cold storage, and those taken from the storage showed 100% regeneration capacity after the passages. A dormancy period lasting about 20 weeks was observed, followed by intensive shoot growth until the 48th week, which led to the exhaustion of the cultures. The changes could be traced to the reduction of the chlorophyll content and the F/F value, as well as in the discoloration of the lower leaves and the appearance of necrotic tissues. Long, etiolated shoots (89.3 mm) were obtained at the end of cold storage. Shoot cultures stored in a growth chamber as control (22 ± 2 °C, 16/8 h light/dark) senesced and died after 16 weeks. Explants from stored shoots were subcultured for four weeks. The number and length of newly developed shoots were significantly higher on explants from cold storage compared to those from control cultures if the storage was longer than one week.
缓慢生长保存可以保护濒危物种的遗传资源，比如该属的那些物种。我们的目的是研究欧洲花楸离体培养物的可保存性、它们的形态生理变化以及在不同储存条件（4 ± 0.5 °C，黑暗；和22 ± 2 °C，16/8小时光照/黑暗）后的再生能力。冷藏持续52周，每四周进行观察。培养物在冷藏下显示100%存活，从储存中取出的培养物在继代培养后显示100%的再生能力。观察到一个持续约20周的休眠期，随后直到第48周芽大量生长，这导致培养物耗尽。这些变化可以追溯到叶绿素含量和F/F值的降低，以及下部叶片变色和坏死组织的出现。冷藏结束时获得了长的黄化芽（89.3毫米）。作为对照储存在生长室（22 ± 2 °C，16/8小时光照/黑暗）中的芽培养物在16周后衰老死亡。将储存芽的外植体继代培养四周。如果储存时间超过一周，与对照培养物的外植体相比，冷藏外植体上新发育芽的数量和长度显著更高。
