Földes-Papp Zeno, Baumann Gerd, Li Long-Cheng
Head of the Department of Geriatrics, Asklepios Clinic Lindau, 88131 Lindau (at Lake Constance), Bavaria, Germany.
Head of the Mathematics Department, Faculty of Basic Sciences, German University in Cairo (GUC), 11835 New Cairo City, Egypt.
J Biol Methods. 2021 Jan 30;8(1):e142. doi: 10.14440/jbm.2021.348. eCollection 2021.
We measured anomalous diffusion in human prostate cancer cells which were transfected with the Alexa633 fluorescent RNA probe and co-transfected with enhanced green fluorescent protein-labeled argonaute2 protein by laser scanning microscopy. The image analysis arose from diffusion based on a "two-level system". A trap was an interaction site where the diffusive motion was slowed down. Anomalous subdiffusive spreading occurred at cellular traps. The cellular traps were not immobile. We showed how the novel analysis method of imaging data resulted in new information about the number of traps in the crowded and heterogeneous environment of a single human prostate cancer cell. The imaging data were consistent with and explained by our modern ideas of anomalous diffusion of mixed origins in live cells. Our original research presented in this study is significant as we obtained a complex diffusion mechanism in live single cells.
我们通过激光扫描显微镜测量了转染了Alexa633荧光RNA探针并共转染了增强型绿色荧光蛋白标记的AGO2蛋白的人前列腺癌细胞中的反常扩散。图像分析基于“两级系统”进行扩散。陷阱是扩散运动减缓的相互作用位点。反常亚扩散在细胞陷阱处发生。细胞陷阱并非固定不动。我们展示了成像数据的新分析方法如何在单个前列腺癌细胞拥挤且异质的环境中产生有关陷阱数量的新信息。成像数据与我们关于活细胞中混合起源反常扩散的现代观点一致并得到了解释。我们在本研究中呈现的原创性研究具有重要意义，因为我们在活的单细胞中获得了复杂的扩散机制。
