Nedoluzhko Artem, Sharko Fedor, Tsygankova Svetlana, Boulygina Eugenia, Ibragimova Amina, Teslyuk Anton, Galindo-Villegas Jorge, Rastorguev Sergey
Faculty of Biosciences and Aquaculture, Nord University, 8049 Bodø, Norway.
Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 119071, Moscow, Russia.
Heliyon. 2021 Feb 8;7(2):e06160. doi: 10.1016/j.heliyon.2021.e06160. eCollection 2021 Feb.
Interspecies hybridization is driven by a complex interplay of factors where introgression plays an important role. In the present study, the transfer of genetic material, between two quite distant fish species from different genera, through spontaneous hybridization was documented with dedicated molecular and bioinformatics tools. We investigate the genomic landscape of putative stickleback-relative introgression by carefully analyzing the tractable transposable elements (TE) on the admixed genome of some individuals of two sympatric stickleback species inhabiting northwestern Russia, namely the three-spined () and the nine-spined () sticklebacks. Our data revealed that unique TE amplification types exist, supporting our proposed hypothesis that infers on the interspecific introgression. By running a restriction site-associated DNA sequencing (RAD-Seq) with eight samples of and and subjecting further the results to a contrasting analysis by variated bioinformatic tools, we identified the related introgression-linked markers. The admixture nature observed in a single sample of the nine-spined stickleback demonstrated the possible traces of remote introgression between these two species. Our work reveals the potential that introgression has on providing particular variants at a high-frequency speed while linking blocks of sequence with multiple functional mutations. However, even though our results are of significant interest, an increased number of samples displaying the introgression are required to further ascertain our conclusions.
种间杂交是由多种因素的复杂相互作用驱动的，其中基因渐渗起着重要作用。在本研究中，利用专门的分子和生物信息学工具记录了来自不同属的两个亲缘关系较远的鱼类物种之间通过自发杂交进行的遗传物质转移。我们通过仔细分析居住在俄罗斯西北部的两种同域棘鱼（即三刺棘鱼（）和九刺棘鱼（））的一些个体的混合基因组上易于处理的转座元件（TE），研究了假定的棘鱼相关基因渐渗的基因组格局。我们的数据表明存在独特的TE扩增类型，支持了我们提出的关于种间基因渐渗的假设。通过对8个三刺棘鱼和九刺棘鱼样本进行限制性位点相关DNA测序（RAD-Seq），并使用各种生物信息学工具对结果进行对比分析，我们确定了与基因渐渗相关的标记。在一个九刺棘鱼样本中观察到的混合性质表明这两个物种之间可能存在远距离基因渐渗的痕迹。我们的工作揭示了基因渐渗在以高频速度提供特定变异同时将序列块与多个功能突变联系起来方面的潜力。然而，尽管我们的结果具有重大意义，但需要增加显示基因渐渗的样本数量以进一步确定我们的结论。
