Department of Gynecology, The First People's Hospital of Wenling, Wenling, Zhejiang, China.
Biosci Biotechnol Biochem. 2021 Feb 18;85(2):280-286. doi: 10.1093/bbb/zbaa016.
Preeclampsia (PE) is characterized by the impaired invasive ability of trophocytes, which can be modulated by microRNAs (miRs). In the current study, the effects of rhynchophylline (Rhy) on the viability and invasive ability of trophocytes were explored by focusing on miR-141-3p/ZEB1 axis. The level of miR-141-3p was modulated in human trophocytes and the changes in cell viability, apoptosis, invasive ability, and ZEB1 level were detected. Then the trophocytes with miR-141-3p overexpression were treated with Rhy and the effects on trophocyte phenotypes were assessed. The induced miR-141-3p level suppressed cell viability, induced apoptosis, and inhibited invasion and ZEB1 level in trophocytes. The treatment of Rhy restored the viability and invasive ability of trophocytes under the overexpression of miR-141-3p, indicating the protective effects of Rhy on trophocytes. The findings in the current study highlighted the protective effects of Rhy on trophocytes during PE progression, which was associated with the inhibition of miR-141-3p.
子痫前期 (PE) 的特征是滋养细胞的浸润能力受损，这可以通过 microRNAs (miRs) 来调节。在本研究中，我们聚焦于 miR-141-3p/ZEB1 轴，探讨了钩藤碱 (Rhy) 对滋养细胞活力和浸润能力的影响。我们调节了人滋养细胞中的 miR-141-3p 水平，并检测了细胞活力、凋亡、浸润能力和 ZEB1 水平的变化。然后，用 Rhy 处理过表达 miR-141-3p 的滋养细胞，并评估其对滋养细胞表型的影响。诱导的 miR-141-3p 水平抑制了滋养细胞的活力，诱导了凋亡，并抑制了浸润和 ZEB1 水平。Rhy 的治疗恢复了 miR-141-3p 过表达下滋养细胞的活力和浸润能力，表明 Rhy 对滋养细胞具有保护作用。本研究的结果强调了 Rhy 在子痫前期进展过程中对滋养细胞的保护作用，这与抑制 miR-141-3p 有关。
