Epigenetics & Neuropsychiatric Disorders Laboratory, CSIR-Centre for Cellular and Molecular Biology (CCMB), Uppal Road, Habsiguda, Hyderabad, Telangana State, 500007, India.
Department of Neurology, Guntur Medical College, Guntur, Andhra Pradesh, 522004, India.
Neuromolecular Med. 2021 Dec;23(4):485-490. doi: 10.1007/s12017-021-08648-0. Epub 2021 Feb 18.
Pharmaco-resistant Epilepsy has been a major challenge for medical interventions in controlling seizures. To date, up to 33% of the patients with epilepsy do not show adequate response to anti-epileptic drugs even after prolonged combinatorial drug usage. Using microarray, this study explores the changes in hippocampal gene expression in the phenytoin-resistant pentylenetetrazol (PTZ)-kindled mouse model of epilepsy. Our results from mRNA microarray analysis show distinct gene expression profiles in the hippocampus of phenytoin-resistant and sensitive mice. Pathway enrichment analysis showed differential expression of genes involved in cholesterol biosynthesis in phenytoin-resistant and sensitive mice.
药物抵抗性癫痫一直是医学干预控制癫痫发作的主要挑战。迄今为止，多达 33%的癫痫患者即使在长期联合用药后也不能对抗癫痫药物有足够的反应。本研究利用微阵列技术，探讨了苯妥英钠耐药戊四氮（PTZ）点燃癫痫小鼠模型中海马基因表达的变化。我们从 mRNA 微阵列分析的结果表明，苯妥英钠耐药和敏感小鼠的海马区有明显不同的基因表达谱。通路富集分析显示，苯妥英钠耐药和敏感小鼠中胆固醇生物合成相关基因的表达存在差异。
