用于 3D 体外组织模型的牛子宫内膜、上皮和基质细胞的分离、培养和鉴定。

Isolation, Culture, and Characterization of Primary Bovine Endometrial, Epithelial, and Stromal Cells for 3D In Vitro Tissue Models.

机构信息

Producción Animal e Industrialización, Facultad de Ingeniería, Universidad Nacional de Chimborazo (UNACH), Riobamba, Ecuador.

Servicio Regional de Investigación y Desarrollo Agroalimentario (SERIDA), Camino de Rioseco 1225, Asturias, Spain.

出版信息

Methods Mol Biol. 2021;2273:103-110. doi: 10.1007/978-1-0716-1246-0_6.

DOI:10.1007/978-1-0716-1246-0_6

PMID:33604847

Abstract

Efficient isolation, characterization, and culture of endometrial epithelial cells and stromal fibroblasts from calf uteri collected at the slaughterhouse is key to develop useful 3D culture tissue models to investigate uterine physiology and pathology without the need of performing invasive procedures to recover tissue samples.Here we provide a detail methodology that gives consistently pure and viable populations of distinct primary bovine endometrial cells.

摘要

从屠宰场采集的牛子宫中高效分离、鉴定和培养子宫内膜上皮细胞和基质成纤维细胞，是开发有用的 3D 培养组织模型的关键，可用于研究子宫生理学和病理学，而无需进行侵入性程序来回收组织样本。本文提供了一种详细的方法，可始终获得纯度高且存活的不同原代牛子宫内膜细胞群体。