Dhankhar Dinesh, Nagpal Anushka, Li Runze, Chen Jie, Cesario Thomas C, Rentzepis Peter M
Department of Electrical and Computer Engineering, Texas A&M University, College Station, USA.
School of Physical Science and Technology, Shanghai Tech University, Shanghai, China.
Appl Spectrosc. 2021 Sep;75(9):1146-1154. doi: 10.1177/0003702821992834. Epub 2021 Feb 19.
The resonance Raman spectra of bacterial carotenoids have been employed to identify bacterial strains and their intensity changes as a function of ultraviolet (UV) radiation dose have been used to differentiate between live and dead bacteria. In addition, the resonance-enhanced Raman spectra enabled us to detect bacteria in water at much lower concentrations (∼10 cells/mL) than normally detected spectroscopically. A handheld spectrometer capable of recording resonance Raman spectra in situ was designed, constructed, and was used to record the spectra. In addition to bacteria, the method presented in this paper may also be used to identify fungi, viruses, and plants, in situ, and detect infections within a very short period of time.
细菌类胡萝卜素的共振拉曼光谱已被用于识别细菌菌株，其强度随紫外线（UV）辐射剂量的变化已被用于区分活细菌和死细菌。此外，共振增强拉曼光谱使我们能够检测水中浓度比正常光谱检测低得多（约10个细胞/毫升）的细菌。设计、构建了一种能够原位记录共振拉曼光谱的手持式光谱仪，并用于记录光谱。除了细菌，本文介绍的方法还可用于原位识别真菌、病毒和植物，并在极短时间内检测感染情况。
