利用同步荧光和主成分分析原位检测经灭活后的活菌与死菌的比例。
In situ detection of live-to-dead bacteria ratio after inactivation by means of synchronous fluorescence and PCA.
机构信息
Department of Electrical and Computer Engineering, Texas A&M University, College Station, TX 77843.
Department of Biological and Agricultural Engineering, Texas A&M University, College Station, TX 77843.
出版信息
Proc Natl Acad Sci U S A. 2018 Jan 23;115(4):668-673. doi: 10.1073/pnas.1716514115. Epub 2018 Jan 8.
Abstract
The determination of live and dead bacteria is of considerable significance for preventing health care-associated infection in hospitals, field clinics, and other areas. In this study, the viable (live) and nonviable (dead) bacteria in a sample were determined by means of their fluorescence spectra and principal component analysis (PCA). Data obtained in this study show that it is possible to identify bacteria strains and determine the live/dead ratio after UV light inactivation and antibiotic treatment, in situ, within minutes. In addition, synchronous fluorescence scans enable the identification of bacterial components such as tryptophan, tyrosine, and DNA. Compared with the time-consuming plating and culturing methods, this study renders a means for rapid detection and determination of live and dead bacteria.
摘要
确定活细菌和死细菌对于预防医院、野外诊所和其他领域的医源性感染具有重要意义。本研究采用荧光光谱和主成分分析（PCA）的方法对样本中的活细菌和死细菌进行了测定。本研究获得的数据表明，有可能在几分钟内在原位鉴定细菌菌株，并确定经紫外线灭活和抗生素处理后的活菌/死菌比例。此外，同步荧光扫描可识别细菌成分，如色氨酸、酪氨酸和 DNA。与耗时的平板和培养方法相比，本研究提供了一种快速检测和确定活细菌和死细菌的方法。
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