Lu Liang, Shang Xifu, Liu Bin, Chen Weijian, Zhang Yu, Liu Shuyun, Sui Xiang, Wang Aiyuan, Guo Quanyi
Department of Orthopaedics, The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui Province, China.
Sports Medicine and Adult Reconstructive Surgery, Nanjing Drum Tower Hospital, Nanjing, Jiangsu Province, China.
J Cell Physiol. 2021 Jun;236(6):4244-4257. doi: 10.1002/jcp.30020. Epub 2021 Feb 19.
The purpose of this study was to investigate the feasibility of adipose-derived stem cells (ADSCs) as the seed cells of cartilage tissue engineering. ADSCs were isolated from adipose tissue that was harvested under sterile conditions from the inguen fold of porcines and cultured in vitro. Acellular cartilage extracellular matrix (ACECM) scaffolds of pigs were then constructed. Moreover, inflammatory cells, as well as cellular and humoral immune responses, were detected using hematoxylin and eosin staining staining, immunohistochemical staining, and western blot analysis. The results showed that the cartilage complex constructed by ADSCs and ACECM through tissue engineering successfully repaired the cartilage defect of the pig knee joint. The in vivo repair experiment showed no significant difference between chondrocytes, ADSCs, and induced ADSCs, indicating that ADSCs do not require in vitro induction and have the potential for chondrogenic differentiation in the environment around the knee joint. In addition, pig-derived acellular cartilage scaffolds possess no obvious immune inflammatory response when used in xenotransplantation. ADSCs may serve as viable seed cells for cartilage tissue engineering.
本研究的目的是探讨脂肪来源干细胞（ADSCs）作为软骨组织工程种子细胞的可行性。ADSCs从猪腹股沟皱襞处无菌采集的脂肪组织中分离出来，并在体外进行培养。然后构建了猪的脱细胞软骨细胞外基质（ACECM）支架。此外，采用苏木精-伊红染色、免疫组织化学染色和蛋白质印迹分析检测炎症细胞以及细胞和体液免疫反应。结果表明通过组织工程由ADSCs和ACECM构建的软骨复合体成功修复了猪膝关节的软骨缺损。体内修复实验表明软骨细胞、ADSCs和诱导后的ADSCs之间无显著差异，这表明ADSCs无需体外诱导，在膝关节周围环境中具有软骨形成分化的潜力。此外，猪源脱细胞软骨支架在异种移植时无明显免疫炎症反应。ADSCs可作为软骨组织工程可行的种子细胞。
