Eötvös Loránd Research Network (ELKH), Biological Research Centre, Szeged, Hungary.
Doctoral School of Biology, University of Szeged, Hungary.
FEBS J. 2021 Aug;288(16):4812-4832. doi: 10.1111/febs.15779. Epub 2021 Mar 5.
Ezrin-Radixin-Moesin (ERM) proteins play an essential role in the cytoplasm by cross-linking actin filaments with plasma membrane proteins. Research has identified the nuclear localization of ERMs, as well as the involvement of a single Drosophila ERM protein, Moesin, in nuclear mRNA exports. However, the question of how important the nuclear activity of ERM proteins are for the life of an organism has so far not been explored. Here, we present the first attempt to reveal the in vivo relevance of nuclear localization of Moesin in Drosophila. With the help of a nuclear export signal, we decreased the amount of Moesin in the nuclei of the animals. Furthermore, we observed various developmental defects, demonstrating the importance of ERM function in the nucleus for the first time. Transcriptome analysis of the mutant flies revealed that the lack of nuclear Moesin function leads to expression changes in nearly 700 genes, among them heat-shock genes. This result together with additional findings revealed that in Drosophila the expression of protein chaperones requires the nuclear functions of Moesin. DATABASE: GEO accession number: GSE155778.
埃兹蛋白-根蛋白-膜突蛋白（ERM）通过将肌动蛋白丝与质膜蛋白交联在细胞质中发挥重要作用。研究已经确定了 ERM 的核定位，以及单个果蝇 ERM 蛋白 Moesin 参与核 mRNA 输出。然而，迄今为止，ERM 蛋白的核活性对生物体的重要性问题尚未得到探索。在这里，我们首次尝试揭示 Moesin 在果蝇中的核定位的体内相关性。借助核输出信号，我们减少了动物细胞核中 Moesin 的数量。此外，我们观察到各种发育缺陷，首次证明了 ERM 功能在核内的重要性。对突变体苍蝇的转录组分析表明，缺乏核 Moesin 功能会导致近 700 个基因的表达变化，其中包括热休克基因。这一结果与其他发现一起表明，在果蝇中，蛋白质伴侣的表达需要 Moesin 的核功能。数据库：GEO 注册号：GSE155778。
