Purification of a novel 30,000 Da calcium-binding protein from bovine cerebellum. - Suppr | 超能文献

Purification of a novel 30,000 Da calcium-binding protein from bovine cerebellum.

作者信息

Kurokawa H, Nonomura Y

机构信息

Department of Pharmacology, Faculty of Medicine, University of Tokyo.

出版信息

J Biochem. 1988 Jan;103(1):8-10. doi: 10.1093/oxfordjournals.jbchem.a122243.

DOI:10.1093/oxfordjournals.jbchem.a122243

Abstract

A novel very acidic calcium-binding protein (CaBP) was purified from bovine cerebellum, using 45Ca autoradiography as a marker, through a preparative procedure involving salting out with a very high concentration of ammonium sulfate, DE52 column chromatography, RNAase treatment, and HPLC gel filtration. This protein showed a molecular weight of 30,0000 dalton (Da) on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and of 120,000 on in gel filtration chromatography analysis under physiological ionic strength. The calcium binding activity of this 30,000 Da CaBP was monitored on the basis of calcium-dependent changes in tyrosine fluorescence (Kd = 3.0 microM).

摘要

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