Isolation of the major O-glycosidically linked oligosaccharides obtained by alkaline borohydride degradation of human meconium glycoproteins.

作者信息

Capon C, Cache P, Leroy Y, Strecker G, Montreuil J, Fournet B

机构信息

Laboratoire de Chimie Biologique, Unité Associée au CNRS No. 217, Université des Sciences et Techniques de Lille Flandres-Artois, Villeneuve d'Ascq, France.

出版信息

J Chromatogr. 1988 Mar 4;425(1):35-45. doi: 10.1016/0378-4347(88)80004-5.

DOI:10.1016/0378-4347(88)80004-5

PMID:3360877

Abstract

Neutral and acidic oligosaccharides derived from human meconium glycoproteins by alkaline borohydride degradation have been separated by high-performance liquid chromatography on a Micro-Pak anion-exchange column. In each class, oligosaccharides were purified by normal-phase (neutral and acidic oligosaccharides) and reversed-phase (neutral oligosaccharides) chromatography. Effective separations of neutral oligosaccharides and acidic oligosaccharides were achieved.

摘要

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