来自印度次大陆的 Acanthogyrus（Acanthosentis）kashmirensis 的分子特征。

The molecular profile of Acanthogyrus (Acanthosentis) kashmirensis from the Indian subcontinent.

机构信息

Cellular and Molecular Research Center, Guilan University of Medical Sciences, Rasht, Iran.

Department of Medical Parasitology and Mycology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran.

出版信息

Acta Parasitol. 2021 Sep;66(3):863-870. doi: 10.1007/s11686-020-00331-x. Epub 2021 Feb 20.

DOI:10.1007/s11686-020-00331-x

PMID:33609238

Abstract

PURPOSE

The purpose of the present study was to provide molecular support for the validity of the morphological description of Acanthosentis kashmirensis Amin, Heckmann, Zargar, 2017 which was originally poorly described as Neoechinorhynchus kashmirensis Fotedar and Dar, 1977 from the Indian subcontinent, and to characterise its molecular identity and phylogenetic relationships.

METHODS

Total DNA was extracted, and the partial region of the small subunit (SSU) 18S rDNA and ITS-rDNA genes were amplified and sequenced. Genetic diversity was calculated and phylogenetic analysis of the nucleotide sequence data was performed.

RESULTS

In this study, the molecular profile of this acanthocephalan was generated for the first time. Based on the partial 18S rDNA, interspecific variation between A. kashmirensis with different species of Acanthosentis and were 3.6-10.3% and 22.4-38.2% based on 18S rDNA and ITS-rDNA genes, respectively. We described the phylogenetic relationships of A. kashmirensis compared with other species of the genus and also with members of the family Quadrigyridae.

CONCLUSIONS

The ITS-rDNA sequences of members of the family Quadrigyridae are more variable than 18S rDNA that can be useful for achieving a proper assessment of biodiversity. Sequence data generation from additional species of Acanthosentis will be needed to reconstruct the phylogenetic relationships of this group of acanthocephalans.

摘要

目的

本研究的目的是为 Amin、Heckmann、Zargar 于 2017 年对 Acanthosentis kashmirensis 的形态描述提供分子支持，该描述最初被描述为 Neoechinorhynchus kashmirensis Fotedar 和 Dar，1977 年来自印度次大陆，并且对其分子身份和系统发育关系进行特征描述。

方法

提取总 DNA，扩增并测序部分小亚基（SSU）18S rDNA 和 ITS-rDNA 基因。计算遗传多样性并对核苷酸序列数据进行系统发育分析。

结果

在本研究中，首次生成了这种棘头虫的分子特征。基于部分 18S rDNA，A. kashmirensis 与不同种的 Acanthosentis 之间的种间差异分别为 3.6-10.3%和 22.4-38.2%，基于 18S rDNA 和 ITS-rDNA 基因。我们描述了 A. kashmirensis 与该属的其他物种以及 Quadrigyridae 科的成员的系统发育关系。