Departamento de Farmacología, Instituto de Farmacología Experimental-IFEC-CONICET-Universidad Nacional de Córdoba, Argentina.
Laboratorio de Neurobiología, Instituto de Investigación Médica Mercedes y Martín Ferreyra-INIMEC-CONICET-Universidad Nacional de Córdoba. Córdoba, Argentina; Instituto Universitario Ciencias Biomédicas Córdoba, Córdoba, Argentina.
Neurobiol Learn Mem. 2021 Mar;179:107409. doi: 10.1016/j.nlm.2021.107409. Epub 2021 Feb 18.
Ghrelin (Gr) is an orexigenic peptide that acts via its specific receptor, GHSR-1a distributed throughout the brain, being mainly enriched in pituitary, cortex and hippocampus (Hp) modulating a variety of brain functions. Behavioral, electrophysiological and biochemical evidence indicated that Gr modulates the excitability and the synaptic plasticity in Hp. The present experiments were designed in order to extend the knowledge about the Gr effect upon structural synaptic plasticity since morphological and quantitative changes in spine density after Gr administration were analyzed "in vitro" and "in vivo". The results show that Gr administered to hippocampal cultures or stereotactically injected in vivo to Thy-1 mice increases the density of dendritic spines (DS) being the mushroom type highly increased in secondary and tertiary extensions. Spines classified as thin type were increased particularly in primary extensions. Furthermore, we show that Gr enhances selectively the expression of BDNF-mRNA species.
生长激素释放肽（Gr）是一种食欲肽，通过其在大脑中广泛分布的特异性受体 GHSR-1a 发挥作用，主要在垂体、皮质和海马体（Hp）中富集，调节多种大脑功能。行为、电生理和生化证据表明，Gr 调节 Hp 中的兴奋性和突触可塑性。本实验旨在扩展关于 Gr 对结构突触可塑性影响的知识，因为 Gr 给药后在形态和密度上的变化进行了分析“体外”和“体内”。结果表明，Gr 给药于海马培养物或立体注射到 Thy-1 小鼠体内，增加树突棘（DS）的密度，蘑菇型在二级和三级延伸中高度增加。被分类为薄型的刺突特别增加在主延伸中。此外，我们还表明，Gr 选择性地增强了 BDNF-mRNA 物种的表达。
